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IRS1 phospho S307 Antibody
Rabbit Polyclonal
600-401-445
100 µg
Liquid (sterile filtered)
WB, ELISA
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-IRS1 pS307 (RABBIT) Antibody - 600-401-445
rabbit anti-IRS1 pS307 antibody, IRS-1, IRS 1, HIRS 1 antibody, HIRS-1 antibody, Insulin Receptor Substrate 1 antibody
Rabbit
Polyclonal
IgG
Target Details
IRS1 - View All IRS1 Products
Human
Phosphorylation
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 290-320 of human IRS1 protein.
This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human IRS1 pS307 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human IRS1 is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with IRS1 from human, mouse, rat, dog and vervet monkey based on 100% homology with the immunizing sequence. Partial reactivity is also expected against IRS1 from pig (94%), bovine (94%) and chicken (88%) sources. Reactivity of this antibody with IRS1 from other species is unknown.
Application Details
ELISA, WB
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to phosphorylated IRS1 protein by western blotting in the appropriate cell lysate or extract. Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS307 of IRS1 protein.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Insulin Receptor Substrate 1 (IRS1) acts as a signaling molecule for IL-4, insulin and insulin-like growth factor-I (IGF-I) receptors. When phosphorylated by the insulin receptor, IRS1 binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. When bound, IRS1 typically activates phosphatidylinositol 3-kinase p85 subunit. IRS1 interacts with both the NPXY motif of tyrosine-phosphorylated IGF1R and the INSR through the PTB domain. Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Diseases associated with IRS1 include Diabetes Mellitus, Noninsulin-Dependent and Mixed Cell Adenoma. Anti-IRS1pS307 is useful for researchers interested in protein kinase binding, IL-2 pathways, and RET signaling.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.