Datasheet

IRAK Antibody [8F1A7]

Name: IRAK Antibody [8F1A7]
Brand: Rockland Immunochemicals
Price: 485.0 USD
Availability: OutOfStock

Mouse Monoclonal [8F1A7] IgG1

Item No. 200-301-BZ2

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: WB, ELISA, IHC

Reactivity: Human, Mouse

Host Species: Mouse

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Specifications for IRAK Antibody [8F1A7]

Product Details

Description: Anti-IRAK (MOUSE) Antibody - 200-301-BZ2

Synonyms: IRAK Antibody [8F1A7] , IRAK, pelle

Host Species: Mouse

Clonality: Monoclonal

Format: IgG1

Target Details

Gene Name: IRAK1 - View All IRAK1 Products

Reactivity: Human, Mouse

Immunogen Type: Recombinant Protein

Immunogen: Anti-IRAK antibody was produced in mice by repeated immunizations with recombinant protein corresponding to amino acids 618 - 712 of human IRAK.

Purity/Specificity: Anti-IRAK Monoclonal Antibody was Protein G purified. Cross reactivity with IRAK [8F1A7] from other sources has not been determined.

Database Links:

P51617 - UniProtKB

P51617 - NCBI Protein

3654 - Gene ID

Application Details

Tested Applications: ELISA, IHC, WB

Application Note: Anti-IRAK Antibody has been tested for use in ELISA, Western Blotting, and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 77 kDa in Western Blots of specific cell lysates and tissues. WB validated in mouse samples and IHC validates in rat samples. All other applications and species not yet tested.

Formulation

Concentration: 1 mg/mL by UV absorbance at 280 nm

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Preservative: 0.02% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-kB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Heat-induced Antigen Retrieval Protocol

Histone Immunoblotting Protocol

Immunohistochemistry (IHC) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Protease-induced Antigen Retrieval Protocol

Sandwich ELISA Protocol for Collagen

Staining Paraffin Sections by PAP Procedure

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.