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Datasheet

Insulin Antibody

Mouse Monoclonal 2D11.H5 IgG1 kappa

200-301-389
100 µg
Liquid (sterile filtered)
ELISA, Dot Blot
Human
Mouse
$485.00 /Per Item
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

Anti-Insulin (MOUSE) Monoclonal Antibody - 200-301-389
mouse anti-insulin antibody, ILPR antibody, INS antibody, Insulin A chain antibody, Insulin B chain antibody, Insulin precursor antibody, IRDN antibody, Proinsulin antibody, Proinsulin precursor antibody
Mouse
Monoclonal
IgG1

Target Details

INS  - View All INS Products
Human
Native Protein
This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).
This protein A purified mouse monoclonal antibody reacts specifically with insulin from human and swine sources.  Cross reactivity with insulin from mouse and rat does not occur.  Cross reactivity with insulin from other sources has not been determined.
P01308 - UniProtKB
P01308.1 - NCBI Protein
3630 - Gene ID

Application Details

Dot Blot, ELISA
Mouse Anti-Insulin Antibody has been tested by ELISA and dot blot and is suitable for immunohistochemistry, immunoblotting and immunoprecipitation. Insulin has a cytoplasmic localization.  Pancreatic tissue or b-cells from islets of Langerhans can be used as a positive control.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Recognizes the 51 amino acid (6 kDa) insulin polypeptide composed of A and B chains. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids.  The main storage site for insulin is the pancreatic islets.  Antibodies to insulin are important as b-cell and tumor (insulinoma) markers.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.