Datasheet

IL-22 Antibody Biotin Conjugated

Name: IL-22 Antibody Biotin Conjugated
Brand: Rockland Immunochemicals
Availability: OutOfStock

Rabbit Polyclonal

Item No. 209-406-J33

Size: 100 µg

Physical State: Lyophilized

Reactivity: Human

Host Species: Rabbit

This product is discontinued

Specifications for IL-22 Antibody Biotin Conjugated

Product Details

Description: Anti-Human IL-22 (RABBIT) Antibody Biotin Conjugated - 209-406-J33

Synonyms: rabbit anti-IL-22 Biotin conjugated antibody, rabbit anti-interleukin-22 biotin conjugated antibody, Interleukin22, IL22, ILTIF, ZCYTO18, IL22, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor

Host Species: Rabbit

Conjugate: Biotin

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: IL22 - View All IL22 Products

Reactivity: Human

Immunogen Type: Recombinant Protein

Immunogen: Anti-IL-22 antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human IL-22 protein from E. coli.

Purity/Specificity: Anti-IL-22 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-22 protein. A BLAST analysis was used to suggest cross-reactivity with IL-22 from human sources based on 100% homology with the immunizing sequence. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum. Cross-reactivity with IL-22 from other sources has not been determined.

Database Links:

Q9GZX6 - UniProtKB

NP_065386.1 - NCBI Protein

gene?db=gene&term=50616 - Gene ID

Application Details

Application Note: IL-22 purified antibody is suitable for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 20 kDa in size corresponding to the human IL-22 protein by western blotting in appropriate cell lysate or extract.

Formulation

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 100 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Interleukin-22 (IL-22) is a member of a group of cytokines called IL-10 family member, produced by activated dendritic cells and T lymphocytes. IL-22 inhibits IL-4 production by Th2 cells, and induces acute phase reactants in the liver and pancreas. IL-22 signals via the IL-22R and IL-10Rß receptors to act as a potent mediator of the cellular inflammatory response. Recombinant human IL-22 is a non-disulfide-linked homodimer with a total molecular weight of 33.6 kDa. Anti-IL-22 antibody is ideal for researchers interested in cytokines and stem cell research.

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.