Mouse Anti-IL-17A Antibody - Western Blot

Datasheet

IL-17A Biotin Conjugated Antibody

Name: IL-17A Biotin Conjugated Antibody
Brand: Rockland Immunochemicals
Price: 495.0 USD
Availability: InStock

Mouse Monoclonal 4H1524.1 IgG2b

Item No. 209-306-B32

Size: 100 µg

Physical State: Lyophilized

Applications: WB

Reactivity: Human

Host Species: Mouse

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Specifications for IL-17A Biotin Conjugated Antibody

Product Details

Description: Anti-Human IL-17A (MOUSE) Biotin Conjugated Monoclonal Antibody - 209-306-B32

Synonyms: mouse anti-Interleukin-17A biotin conjugated antibody, mouse anti-IL-17A biotin conjugated antibody, IL-17A, Interleukin-17A, Cytotoxic T-Lymphocyte-associated Antigen 8, CTLA8, Interleukin-17, IL17, IL-17, Interleukin17

Host Species: Mouse

Conjugate: Biotin

Clonality: Monoclonal

Format: IgG2b

Target Details

Gene Name: IL17A - View All IL17A Products

Reactivity: Human

Immunogen Type: Recombinant Protein

Immunogen: Anti-IL-17A (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17A produced in E.coli followed by hybridoma development.

Purity/Specificity: This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17A protein. A BLAST analysis was used to suggest cross-reactivity with IL-17A from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17A from other sources has not been determined.

Database Links:

Q16552 - UniProtKB

NP_002181.1 - NCBI Protein

Application Details

Tested Applications: WB

Application Note: This purified antibody has been tested by Western Blot and suitable for use in FLOW. Specific conditions for reactivity should be optimized by the end user.

Formulation

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 100 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

IL17-A (also known as Interleukin-17) is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. IL17-A is the founding member of a group of cytokines called the IL17 family. IL17-A was originally identified as a transcript from a rodent T-cell hybridoma. To elicit its functions, IL17 binds to a type I cell surface receptor called IL17R of which there are at least three variants IL17RA, IL17RB, and IL17RC. Anti-IL-17A antibody is ideal for investigators involved in Immunology research.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.