Biotin Rabbit Anti Rat IL-17A Antibody - Western Blot

Datasheet

IL-17A Antibody Biotin Conjugated

Name: IL-17A Antibody Biotin Conjugated
Brand: Rockland Immunochemicals
Price: 455.0 USD
Availability: InStock
Rating: 5 (1 reviews)

Rabbit Polyclonal

1 References

Item No. 212-406-B32

Size: 100 µg

Physical State: Lyophilized

Applications: WB, ELISA

Reactivity: Mouse, Rat

Host Species: Rabbit

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Specifications for IL-17A Antibody Biotin Conjugated

Product Details

Description: Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated - 212-406-B32

Synonyms: rabbit anti-IL-17A biotin Conjugated Antibody, rabbit anti-Interleukin-17A biotin Conjugated Antibody, Interleukin17, IL-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8

Host Species: Rabbit

Conjugate: Biotin

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: Il17a - View All Il17a Products

Reactivity: Mouse, Rat

Immunogen Type: Recombinant Protein

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL17-A protein.

Purity/Specificity: This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Database Links:

Q61453 - UniProtKB

Q61453.1 - NCBI Protein

301289 - Gene ID

Application Details

Tested Applications: WB

Suggested Applications: ELISA - View References

Application Note: This purified antibody has been tested for use in western blotting and suitable in ELISA. By western blot a band approximately 30.0 kDa in size corresponding to rat IL17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Formulation

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 100 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Rat IL17-A (also known as Interleukin-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8) is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Rat IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58% identity between the amino acid sequence of human and rat IL-17A. Anti-IL-17A antibody is ideal for investigators involved in Immunology research.

References (1)

Ayyaswamy S et al. (2023). Obstructive Sleep Apnea-Induced Hypertension Is Associated With Increased Gut and Neuroinflammation. J Am Heart Assoc.

Applications

EIA, ELISA

PubMed

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.