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IKK E phospho T501 Antibody
Rabbit Polyclonal
3 References
600-401-267
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-IKKε pT501 (RABBIT) Antibody - 600-401-267
rabbit anti-IKK epsilon pT501 antibody, rabbit anti-IKKE pT501 antibody, I kappa B kinase epsilon antibody, IKK-epsilon, IKK-E, Inducible I kappa-B kinase, IkBKE antibody, IKBKE protein antibody, Inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, IKKE, IKKI
Rabbit
Polyclonal
IgG
Target Details
IKBKE - View All IKBKE Products
Human
Phosphorylation
Conjugated Peptide
IKKe phospho peptide corresponding to a region of the human protein surrounding pT501 conjugated to KLH.
Anti-IKKε pT501 antibody was affinity purified from monospecific antiserum by immunoaffinity purification against the phosphopeptide and cross adsorption against the non-phosphorylated form of the peptide followed by non-adsorption against a non-specific peptide backbone to further reduce non-specific reactivity. This phospho specific polyclonal antibody is specific for phosphorylated pT501 human IKKe. Reactivity with non-phosphorylated IKKe is minimal. Cross reactivity with pT501 phosphorylated IKKe from mouse, rat or other species has not been determined.
Application Details
ELISA, WB
IHC
- View References
IKKε pT501 antibody is tested in ELISA, western blotting, and although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. An 85 kDa band corresponding to human IKKe is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Researchers should determine optimal titers for other applications.
Formulation
1 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.1% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKepsilon is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFα or IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa. Anti-IKKε pT501 antibody is ideal for investigators involved in NFkappaB and apoptosis research.
DeDiego ML et al. (2019). Interferon-induced protein 44 interacts with cellular FK506-binding protein 5, negatively regulates host antiviral responses, and supports virus replication. mBio.
Applications
WB, IB, PCA
DeDiego ML et al. (2019). Novel functions of IFI44L as a feedback regulator of host antiviral responses. J Virol.
Applications
WB, IB, PCA
Sweeney SE, Mo L, Firestein GS. (2007). Antiviral gene expression in rheumatoid arthritis: role of IKKepsilon and interferon regulatory factor 3. Arthritis Rheum.
Applications
WB, IB, PCA; IHC, ICC, Histology
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.