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IKK alpha Antibody
Mouse Monoclonal 14A231 IgG1 kappa
200-301-H82
100 µg
Liquid
WB, IHC, IF, FC, ChIP, IP
Human
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-IKKα (MOUSE) Monoclonal Antibody - 200-301-H82
IKKA, TCF16, Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, 2.7.11.10, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, IKK1, Nuclear factor NF-kappa-B inhibitor kinase alpha, NFKBIKA, Transcription factor 16, TCF-16
Mouse
Monoclonal
IgG1
Target Details
CHUK - View All CHUK Products
Human
Recombinant Protein
IKK alpha Antibody was produced in mice prepared by repeated immunizations by a His-tagged human IKK alpha protein at full-length.
Anti-IKKα Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with IKKα from Human, Mouse and New World Monkey based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-IKKα from other sources has not been determined.
Application Details
ChIP, FC, IF, IHC, IP, WB
Anti-IKK alpha antibody is tested for use in WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, and IP. Expect a band approximately 85-90kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.05% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-IKK alpha antibody detects human IKK alpha. NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK alpha, IKK beta, and IKK gamma. In vitro, IKK alpha and IKK beta can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK alpha and IKK beta are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Targeted disruption of IKK alpha gene in mice results in skin and limb abnormalities and death of newborns. Anti-IKK alpha antibody is ideal for investigators involved in cytokines and growth factor and NFkappaB research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
