Datasheet

IKB alpha Antibody

Name: IKB alpha Antibody
Brand: Rockland Immunochemicals
Price: 549.0 USD
Availability: OutOfStock

Mouse Monoclonal 6A920 IgG1 kappa

Item No. 200-301-H80

Size: 100 µg

Physical State: Liquid

Applications: WB, IHC, IF, FC, ChIP, IP

Reactivity: Human, Mouse

Host Species: Mouse

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Specifications for IKB alpha Antibody

Product Details

Description: Anti-IKBα (MOUSE) Monoclonal Antibody - 200-301-H80

Synonyms: IKBA, MAD3, NFKBI, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3

Host Species: Mouse

Clonality: Monoclonal

Format: IgG1

Target Details

Gene Name: NFKBIA - View All NFKBIA Products

Reactivity: Human, Mouse

Immunogen Type: Recombinant Protein

Immunogen: IkB alpha Antibody was produced in mice prepared by repeated immunizations of recombinant protein corresponding to internal amino acids in the human IkB alpha protein.

Purity/Specificity: Anti-IkBα Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with IkBα from Human and Mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-IkBα from other sources has not been determined.

Database Links:

P25963 - UniProtKB

NP_065390.1 - NCBI Protein

gene?db=gene&term=4792 - Gene ID

Application Details

Tested Applications: ChIP, FC, IF, IHC, IP, WB

Application Note: Anti-IkB alpha antibody is tested for use in WB, Flow, ICC/IF, IHC, IHC-P, and IP. Expect a band approximately 35kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Formulation

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.05% (w/v) Sodium Azide

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Anti-IkB alpha detects human IkB alpha. NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkBa. Anti-IkB alpha antibody is ideal for investigators involved in NFkappaB, kinase and phosphatase research.

Related Protocols

Adherent Cell Lysis Protocol

Bifunctional Cross-Linking Protocol

Chromatin Immunoprecipitation (ChIP) Protocol

Flow Cytometry (FC) Protocol

Fluorescent Western Blotting Protocol

Heat-induced Antigen Retrieval Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Immunohistochemistry (IHC) Protocol

Immunoprecipitation (IP) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Nucleosome ChIP Protocol

Protease-induced Antigen Retrieval Protocol

Re-ChIP Protocol

Staining Paraffin Sections by PAP Procedure

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.