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IKB alpha Antibody
Rabbit Polyclonal
4 References
100-4167C
100 µL
Liquid (sterile filtered)
WB, EMSA
Human, Mouse, Rat
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-IKBα C-terminal (RABBIT) Antibody - 100-4167C
rabbit anti-IKB alpha Antibody, IkappaBalpha antibody, IKBA antibody, IKBalpha antibody, MAD3 antibody, NF kappa B inhibitor alpha antibody, NFKBI antibody, NFKBIA antibody
Rabbit
Polyclonal
Antiserum
Target Details
NFKBIA - View All NFKBIA Products
Human, Mouse, Rat
Conjugated Peptide
IKB alpha Antibody was produced by repeated immunizations with a synthetic IkBa peptide corresponding to a region near the C-terminus of the human protein.
IKB alpha Antibody was prepared from monospecific antiserum by delipidation and defibrination. Anti- IkBa may react non-specifically with other proteins. Control peptide (code #100-4167P) will compete only with the specific reaction of antiserum with IkBa.
Application Details
WB
EMSA
- View References
Anti-IkBa Antibody has been tested by western blot and is suitable for ELISA, and IHC. Researchers should determine optimal titers for applications that are not stated below.
Formulation
80 mg/mL by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.1% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-IKB alpha Antibody detects IkBa. I-kappa-B-alpha inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, IKBA becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. Anti-IKBa Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction and Immunology research.
Wan YY et al. (2003). The survival of antigen-stimulated T cells requires NFκB-mediated inhibition of p73 expression. Immunity.
Applications
WB, IB, PCA
Cheshire JL et al. (1999). Involvement of Double-stranded RNA-activated Protein Kinase in the Synergistic Activation of Nuclear Factor-κB by Tumor Necrosis Factor-α and γ-Interferon in Preneuronal Cells. J Biol Chem.
Applications
WB, IB, PCA
Miller WE et al. (1998). Interaction of tumor necrosis factor receptor-associated factor signaling proteins with the latent membrane protein 1 PXQXT motif is essential for induction of epidermal growth factor receptor expression Mol Cell Biol
Applications
WB, IB, PCA
Cheshire JL et al. (1997). Synergistic activation of NF-kappaB by tumor necrosis factor alpha and gamma interferon via enhanced I kappaB alpha degradation and de novo I kappaBbeta. Mol Cell Biol
Applications
Supershift, Gel Shift, EMSA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
