Human Red Blood Cell RBC Antibody
Rabbit Polyclonal
7 References
109-4139
2 mL
Lyophilized
FC, Agglutination, Cellular Assay
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Human Red Blood Cell (RBC) (RABBIT) Antibody - 109-4139
rabbit Anti-human RBC antibody, Red blood cell antibody, Antibody for hemagglutination, rabbit anti-RBC, rabbit antibody to human red blood cells (RBC), haemolysin, hemolysin, erythrocytes sensitizing agent, anti-erythrocytes, anti-erythrocytes antibody
Rabbit
Polyclonal
Antiserum
Target Details
Human washed pooled Red Blood Cells (RBC)
This product was prepared from polyspecific antiserum by a delipidation and defibrination.
Application Details
Agglutination
Cellular Assay, FC
- View References
Anti-Human Red Blood Cells Antibody has been tested by agglutination and is suitable for sensitizing cells in hemolytic complement assays, and for lymphocyte screening procedures.
Tissue Data
Red Blood Cells
Formulation
Non-sterile
85 mg/mL by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
2.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Human Red Blood Cells Antibody recognizes human red blood cells and can be used in a variety of agglutination assays where agglutination or clumping of red blood cells is a positive indication of the presence of an analyte, virus particle or bacteria. Red blood cells (RBCs), also known as erythrocytes, are metabolically active cells that are highly adapted to serve their function in blood gas exchange (oxygen/CO2 transport). The red blood cells enable the transport of sufficient O2 between respiratory surfaces (lungs, gills) and metabolizing tissues by means of their high intracellular concentration of hemoglobin.
Hayes BH et al. (2023). Confinement plus myosin-II suppression maximizes heritable loss of chromosomes, as revealed by live-cell ChReporters. J Cell Sci.
Applications
FC, FACS, FLOW
Caielli S. et al. (2021). Erythroid mitochondrial retention triggers myeloid-dependent type I interferon in human SLE. Cell.
Applications
Phagocytosis Assay
Hayes BH. et al. (2020). Macrophages show higher levels of engulfment after disruption of cis interactions between CD47 and the checkpoint receptor SIRPα. Journal of Cell Science
Applications
Other
Steinkuhler J et al. (2018). Membrane fluctuations and acidosis regulate cooperative binding of 'marker of self'protein CD47 with the macrophage checkpoint receptor SIRPα. J Cell Sci.
Applications
Ab Opsonization
Alvey et al. (2017). SIRPA-Inhibited, Marrow-Derived Macrophages Engorge, Accumulate, and Differentiate in Antibody-Targeted Regression of Solid Tumors. Current Biology
Applications
Ab Opsonization
Akutsu H et al. (2015). Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells. Regen Ther.
Applications
Inner Cell Mass (ICM)
Chen AE et al. (2007). Derivation of human embryonic stem cells by immunosurgery. J Vis Exp.
Applications
Inner Cell Mass (ICM)
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
