Human Red Blood Cell Antibody
Rabbit Polyclonal
7 References
209-4139
50 mg
Lyophilized
WB, IHC, FC, Agglutination, Biochemical Assay, LFA
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Human Red Blood Cell (RBC) (RABBIT) Antibody - 209-4139
rabbit Anti-human RBC antibody, Red blood cell antibody, Antibody for hemagglutination, rabbit anti-human red blood cell, rabbit antibody to human red blood cells (RBC), haemolysin, hemolysin, erythrocytes sensitizing agent, anti-erythrocytes, anti-erythrocytes antibody
Rabbit
Polyclonal
IgG
Target Details
Human
Human washed pooled Red Blood Cells (RBC)
This product is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Application Details
Agglutination, IHC
Biochemical Assay, FC, WB, LFA
- View References
Anti-Human Red Blood Cells Antibody has been tested by agglutination and immunohistochemistry. This product is suitable for sensitizing cells in hemolytic complement assays, and for lymphocyte screening procedures, especially screening for T cells and T receptor-bearing lymphocytes. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers. Specific conditions should be optimized by researcher.
Tissue Data
Red Blood Cells
Formulation
Non-sterile
10 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
5.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Human Red Blood Cells Antibody recognizes human red blood cells and can be used in a variety of agglutination assays where agglutination or clumping of red blood cells is a positive indication of the presence of an analyte, virus particle or bacteria. Red blood cells (RBCs), also known as erythrocytes, are metabolically active cells that are highly adapted to serve their function in blood gas exchange (oxygen/CO2 transport). The red blood cells enable the transport of sufficient O2 between respiratory surfaces (lungs, gills) and metabolizing tissues by means of their high intracellular concentration of hemoglobin.
Andrechak JC et al. (2022). CD47-SIRPα Checkpoint Disruption in Metastases Requires Tumor-Targeting Antibody for Molecular and Engineered Macrophage Therapies. Cancers (Basel).
Applications
Immunization
Dick JK et al. (2022). Natural Killer Cell Antibody-Dependent Cellular Cytotoxicity (ADCC) Activity Against Plasmodium falciparum-Infected Red Blood Cells. Methods Mol Biol.
Applications
Undefined
Bockermann R et al. (2021). Imlifidase-generated Single-cleaved IgG: Implications for Transplantation. Transplantation.
Applications
WB, IB, PCA
SCHVIND PITER, CAESAR ARIANE (2020). DEVICE AND METHOD FOR DETECTING BLOOD GROUPS ANTIGENS USING AN INCOMPLETE ANTIBODY. eLibrary, invention patent.
Applications
Lateral Flow (LFA)
Arora G et al. (2018). NK cells inhibit Plasmodium falciparum growth in red blood cells via antibody-dependent cellular cytotoxicity. eLife
Applications
FC, FACS, FLOW; Other
Sasank Vemulapati et al. (2018). H.E.R.M.E.S: rapid blood-plasma separation at the point-of-need. Lab Chip.
Applications
Lateral Flow (LFA)
Järnum S et al. (2017). Enzymatic Inactivation of Endogenous IgG by IdeS Enhances Therapeutic Antibody Efficacy. Mol Cancer Ther.
Applications
Opsonophagocytosis Assay
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
