Datasheet

Hsp40 (YDJ1) Antibody

Name: Hsp40 (YDJ1) Antibody
Brand: Rockland Immunochemicals
Price: 485.0 USD
Availability: OutOfStock

Mouse Monoclonal 1G10.H8 IgG1 kappa

Item No. 200-301-E58

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: WB, IP

Reactivity: Yeast

Host Species: Mouse

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Specifications for Hsp40 (YDJ1) Antibody

Product Details

Description: Anti-HSP40 (YDJ1) (MOUSE) Monoclonal Antibody - 200-301-E58

Synonyms: Mitochondrial protein import protein MAS5, Yeast dnaJ protein 1, YDJ1, MAS5, YNL064C, N2418, YNL2418C

Host Species: Mouse

Clonality: Monoclonal

Format: IgG1

Target Details

Gene Name: YDJ1 - View All YDJ1 Products

Reactivity: Yeast

Immunogen Type: Recombinant Protein

Immunogen: Hsp40 (YDJ1) Antibody was produced in mice by repeated immunizations raised against full length protein yeast Hsp40 (YDJ1).

Purity/Specificity: Anti-Hsp40, YDJ1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Hsp40 from yeast based on 100% homology with the immunizing sequence. Cross-reactivity with Hsp40 from other sources has not been determined. Heat Shock research.

Database Links:

P25491 - UniProtKB

NP_014335.1 - NCBI Protein

Application Details

Tested Applications: IP, WB

Application Note: Anti-Hsp40 Antibody has been tested by western blot and IP and is suitable for ELISA. Expect a band approximately ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Formulation

Concentration: 1 mg/mL by UV absorbance at 280 nm

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: 50% (v/v) Glycerol

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. There are two main types of Hsp40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding, type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins. The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

Immunoprecipitation (IP) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.