Datasheet

HSP22 Antibody

Name: HSP22 Antibody
Brand: Rockland Immunochemicals
Price: 445.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 100-401-F54

Size: 100 µL

Physical State: Liquid (sterile filtered)

Applications: WB, IHC, IF, IP

Reactivity: Human, Mouse, Rat

Host Species: Rabbit

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Specifications for HSP22 Antibody

Product Details

Description: Anti-HSP22 (RABBIT) Antibody - 100-401-F54

Synonyms: Alpha crystallin C chain, CMT2L, CRYAC, DHMN2, H11, Heat shock 22kDa protein 8, HMN2, HSB8, HspB8, Heat shock protein beta-8, E2IG1, HSP22, E2-induced gene 1 protein, Protein kinase H11

Host Species: Rabbit

Clonality: Polyclonal

Format: Antiserum

Target Details

Gene Name: HSPB8 - View All HSPB8 Products

Reactivity: Human, Mouse, Rat

Immunogen Type: Recombinant Protein

Immunogen: Hsp22 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against human Hsp22.

Purity/Specificity: Anti-Hsp22 Antibody was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest cross-reactivity with Hsp22 from Human, Mouse, and Rat based on 100% homology with the immunizing sequence. Not yet tested on other species. Does not cross-react with Hsp27 or alpha-crystallin. Cross-reactivity with Hsp22 from other sources has not been determined. Heat Shock research.

Database Links:

Q9UJY1 - UniProtKB

NP_055180.1 - NCBI Protein

Application Details

Tested Applications: IF, IHC, IP, WB

Application Note: Anti-Hsp22 Antibody has been tested in WB, IF, IHC, and IP. Expect a band approximately ~22kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Formulation

Concentration: 85 mg/mL by Refractometry

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27. HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs. The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macroautophagy. Mutations in the HSPB8 gene are associated with the inherited peripheral neurpathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L).

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Heat-induced Antigen Retrieval Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Immunohistochemistry (IHC) Protocol

Immunoprecipitation (IP) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Protease-induced Antigen Retrieval Protocol

Staining Paraffin Sections by PAP Procedure

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.