![Histone H4 [Dimethyl Lys20] Immunofluorescence](https://rocklandwebimagescdn.azureedge.net/webimages/600-401-J02-HistoneH4-K20me2-Antibody-1-IF-4x3.jpg "Histone H4 [Dimethyl Lys20] Immunofluorescence")
![Histone H4 [Dimethyl Lys20] Western Blot](https://rocklandwebimagescdn.azureedge.net/webimages/600-401-J02-HistoneH4-K20me2-Antibody-2-WB-2x5.jpg "Histone H4 [Dimethyl Lys20] Western Blot")
![Histone H4 [Dimethyl Lys20] ChIP](https://rocklandwebimagescdn.azureedge.net/webimages/600-401-J02-HistoneH4-K20me2-Antibody-3-ChIP-4x3.jpg "Histone H4 [Dimethyl Lys20] ChIP")
![Histone H4 [Dimethyl Lys20] Western Blot](https://rocklandwebimagescdn.azureedge.net/webimages/600-401-J02-HistoneH4-K20me2-Antibody-4-WB-2x5.jpg "Histone H4 [Dimethyl Lys20] Western Blot")
![Histone H4 [Dimethyl Lys20] Dot Blot](https://rocklandwebimagescdn.azureedge.net/webimages/600-401-J02-HistoneH4-K20me2-Antibody-5-DB-4x3.jpg "Histone H4 [Dimethyl Lys20] Dot Blot")
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Histone H4 K20me2 Antibody
Rabbit Polyclonal
600-401-J02
50 µg
Liquid (sterile filtered)
WB, IF, ChIP, Dot Blot
Human, C. elegans
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Histone H4 [Dimethyl Lys20] (RABBIT) Antibody - 600-401-J02
rabbit anti-Histone H4 dimethyl Lys20 antibody, H4K20me2, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4
Rabbit
Polyclonal
IgG
Target Details
HIST4H4 - View All HIST4H4 Products
Human, C. elegans
Methylation
Conjugated Peptide
Histone H4 [Dimethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 20 of human Histone H4.
Anti-Histone H4 [Dimethyl Lys20] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.
Application Details
ChIP, Dot Blot, IF, WB
Anti-Histone H4 [Dimethyl Lys20] antibody is tested for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
Formulation
0.53 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
30% Glycerol
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
When K20 is di-methylated on H4, the downstream effect is silencing of genes. This modification is also necessary and sufficient for 53BP1 binding, which is a prerequisite for DNA repair, a highly conserved mechanism. H4K20me2 is associated with hypoacetylation, and inactivation of certain genes. NSD1 and Suv420h1/2 trigger the dimethylation of K20, but only after SET8 catalyzes the mono-methylation of the same lysine, indicating a complex regulation of this modification that can so seriously affect replication and stability of genomic information. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, DNA Repair, DNA replication Transcription Translation and Splicing, Histones and Modified Histones, and Epigenetics research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.