![Histone H4 [Monomethyl Lys20] Immunofluorescence](https://rocklandwebimagescdn.azureedge.net/webimages/600-401-J01-HistoneH4-K20me1-Antibody-1-IF-4x3.jpg "Histone H4 [Monomethyl Lys20] Immunofluorescence")
![Histone H4 [Monomethyl Lys20] Western Blot](https://rocklandwebimagescdn.azureedge.net/webimages/600-401-J01-HistoneH4-K20me1-Antibody-2-WB-2x5.jpg "Histone H4 [Monomethyl Lys20] Western Blot")
![Histone H4 [Monomethyl Lys20] ChIP](https://rocklandwebimagescdn.azureedge.net/webimages/600-401-J01-HistoneH4-K20me1-Antibody-3-ChIP-4x3.jpg "Histone H4 [Monomethyl Lys20] ChIP")
![Histone H4 [Monomethyl Lys20] Western Blot](https://rocklandwebimagescdn.azureedge.net/webimages/600-401-J01-HistoneH4-K20me1-Antibody-4-WB-2x5.jpg "Histone H4 [Monomethyl Lys20] Western Blot")
![Histone H4 [Monomethyl Lys20] Dot Blot](https://rocklandwebimagescdn.azureedge.net/webimages/600-401-J01-HistoneH4-K20me1-Antibody-5-DB-4x3.jpg "Histone H4 [Monomethyl Lys20] Dot Blot")
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Histone H4 K20me1 Antibody
Rabbit Polyclonal
1 References
600-401-J01
50 µg
Liquid (sterile filtered)
WB, IF, ChIP, Dot Blot, Multiplex
Human, Mouse, C. elegans
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Histone H4 [Monomethyl Lys20] (RABBIT) Antibody - 600-401-J01
rabbit anti-Histone H4 monomethyl Lys20 antibody, H4K20me1, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4
Rabbit
Polyclonal
IgG
Target Details
HIST4H4 - View All HIST4H4 Products
Human, Mouse, C. elegans
Methylation
Conjugated Peptide
Histone H4 [Monomethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 20 of human Histone H4.
Anti-Histone H4 [Monomethyl Lys20] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.
Application Details
ChIP, Dot Blot, IF, WB, Multiplex
Anti-Histone H4 [Monomethyl Lys20] antibody is tested for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
Formulation
1.12 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
30% Glycerol
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
SET8 specifically catalyzes the mono-methylation of H4 at K20. Loss of this crucial methylation causes multiple DNA breaks, which instigates a p53-dependent DNA damage response to avoid mitosis and aberrant chromosomal activity. Therefore, this PTM is essential to genome replication and stability through S-phase. In mammalian stem cells, Xist expression blocks the formation of H4K20me1, which is one of the first examples of a direct connection between chromatin and stem cell differentiation. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, DNA Repair, DNA replication Transcription Translation and Splicing, Histones and Modified Histones, and Epigenetics research.
Moiseeva et al. (2017). ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1. Nature Communications
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.