![Histone H4 [ac Lys16] Immunofluorescence](https://rocklandwebimagescdn.azureedge.net/webimages/600-401-J00-HistoneH4-K16ac-Antibody-1-IF-4x3.jpg "Histone H4 [ac Lys16] Immunofluorescence")
![Histone H4 [ac Lys16] Western Blot](https://rocklandwebimagescdn.azureedge.net/webimages/600-401-J00-HistoneH4-K16ac-Antibody-2-WB-2x5.jpg "Histone H4 [ac Lys16] Western Blot")
![Histone H4 [ac Lys16] Dot Blot](https://rocklandwebimagescdn.azureedge.net/webimages/600-401-J00-HistoneH4-K16ac-Antibody-3-DB-4x3.jpg "Histone H4 [ac Lys16] Dot Blot")
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Histone H4 K16ac Antibody
Rabbit Polyclonal
600-401-J00
50 µg
Liquid (sterile filtered)
WB, IF, Dot Blot, Multiplex
Human, Mouse
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Histone H4 [ac Lys16] (RABBIT) Antibody - 600-401-J00
rabbit anti-Histone H4 Ac Lys16 antibody, H4K16ac, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4
Rabbit
Polyclonal
IgG
Target Details
HIST4H4 - View All HIST4H4 Products
Human, Mouse
Acetylation
Conjugated Peptide
Histone H4 [ac Lys16] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 16 of human Histone H4.
Anti-Histone H4 [ac Lys16] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.
Application Details
Dot Blot, IF, WB, Multiplex
Anti-Histone H4 [ac Lys16] antibody is tested for Western Blot, Dot Blot, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
Formulation
1.0 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys16 (H4 K16Ac) plays a role in transcriptional activation and DNA repair. The protein MOF (MYST1) is the primary enzyme to acetylate K16, along with Gcn5, Esal, ATF2, and Sas2. Depletion of MOF reduces K16 acetylation, causing cell cycle arrests, chromosomal aberrations, and a poor DNA damage response. Anti-Histone H4 are ideal for researchers interested in Epigenetics, HA Epitope Tags, Chromatin Modifiers, Chromatin Research, Histones and Modified Histones research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.