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Hepatitis Virus A59 Nonstructural Protein 9 Antibody
Mouse Monoclonal 2C6.H1 IgG2b kappa
1 References
200-301-A56
100 µg
Liquid (sterile filtered)
WB, IF
Mouse
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Hepatitis Virus (Strain A59) Nonstructural Protein 9 (nsp9) (MOUSE) Monoclonal Antibody - 200-301-A56
mouse anti-Hepatitis Virus A59 Nonstructural Protein 9 Antibody, mouse anti-MHV-A59 nsp9 protein antibody
Mouse
Monoclonal
IgG2b
Target Details
1a - View All 1a Products
Mouse
Native Protein
This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
This antibody is directed against the MHV-A59 nsp9 protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. No cross reactivity occurs with SARS CoV nsp9. Cross reactivity with homologues from other sources has not been tested.
Application Details
IF, WB
This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3% PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.
Formulation
2.112 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
Malireddi, R.K.S., Bynigeri, R.R., Mall, R. et al. (2023). Inflammatory cell death, PANoptosis, screen identifies host factors in coronavirus innate immune response as therapeutic targets. Commun Biol
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.