HDAC3 Antibody
Immunofluorescence Microscopy of anti-HDAC3 antibody
Chromatin Immunoprecipitation for Anti-HDAC3 antibody
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Datasheet

HDAC3 Antibody

Mouse Monoclonal IgG1

200-301-V03
50 µg
Liquid (sterile filtered)
IF, ChIP
Human
Mouse
This product is discontinued

Product Details

Anti-HDAC3 (MOUSE) Monoclonal Antibody - 200-301-V03
HD3, RPD3-2, SMAP45
Mouse
Monoclonal
IgG1

Target Details

HDAC3  - View All HDAC3 Products
Human
Conjugated Peptide
Anti-HDAC3 Antibody was produced in mice by repeated immunization with a synthetic peptide containing a sequence from the C-terminal region of the human HDAC3 protein.
Anti-HDAC3 Antibody was purified by Protein A chromatography. Cross-reactivity with HDAC3 from other sources has not been determined.
O15379 - UniProtKB
NP_003874.2 - NCBI Protein
8841 - Gene ID

Application Details

ChIP, IF
Anti-HDAC3 Antibody is tested for Chromatin Immunoprecipitation and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 48.8 kDa in the appropriate cell lysate or extract.

Formulation

2 mg/ml by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.05% (w/v) Sodium Azide and 0.05% ProClin 300
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
HDAC3 catalyses the deacetylation of lysine residues in the N-terminal part of the core histones (H2A, H2B, H3 and H4). Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression and HDAC activity is associated with gene repression. Histone deacetylation is established by the formation of large multiprotein complexes. HDAC3 may bind to the zinc-finger transcription factor YY1, thereby regulating transcription. It is also able to modulate cell growth and apoptosis through the interaction with p53 and is thought to be essential for the repression of the POU1F1 transcription factor. Anti-HDAC3 Antibody is ideal for research in Epigenetics and Gene Expression.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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