Datasheet

HDAC2 Phospho S394 Antibody

Name: HDAC2 Phospho S394 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 600-401-X11

Size: 100 µL

Physical State: Liquid (sterile filtered)

Applications: WB

Reactivity: Mouse, Rat

Host Species: Rabbit

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Specifications for HDAC2 Phospho S394 Antibody

Product Details

Description: Anti-HDAC2 pS394 (RABBIT) Antibody - 600-401-X11

Synonyms: Histone Deacetylase 2, HD2

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: HDAC2 - View All HDAC2 Products

Reactivity: Mouse, Rat

PTM Specificity: Phosphorylation

Immunogen Type: Conjugated Peptide

Immunogen: Anti-Phospho-Ser394 HDAC2 Antibody was produced in rabbits by repeated immunizations with a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser394 of human HDAC2.

Purity/Specificity: Anti-Phospho-Ser394 HDAC2 Antibody is directed against HDAC2 protein phosphorylated at S394. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated HDAC2. Minimal reactivity occurs against non-phosphorylated HDAC2. Reactivity is completely blocked by treatment with lambda-phosphatase. Cross reactivity is expected against bovine, chicken, guinea pig, human, non-human primates, rat, and sheep.

Database Links:

Q92769 - UniProtKB

NP_001518.3 - NCBI Protein

3066 - Gene ID

Application Details

Tested Applications: WB

Application Note: Anti-Phospho-Ser394 HDAC2 Antibody has been tested by Western Blots and is specific for the ~55 kDa HDAC2 protein phosphorylated at Ser394 in Western blots. Immunolabeling is completely blocked by lambda-Phosphatase treatment (30 minutes, 800units/1mg protein). Specific conditions for reactivity should be optimized by the end user.

Formulation

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Preservative: None

Stabilizer: 0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Histone Deacetylase 2 (HDAC2) is part of a family of histone deacetylases that are responsible for deacetylation of lysine residues in the histone core. HDAC2 is classified as a class I histone deacetylase and is ubiquitously expressed throughout the body. It has been shown that HDAC2 plays an important role in cardiac hypertrophy. Phosphorylation of ser394 is responsible for the hypertrophy-associated activation of HDAC2, whereas intrinsic basal activity is maintained by phosphorylation of ser422 and ser424. HDAC2 pS394 Antibody is ideal for researchers interested in neuroscience research.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.