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GST Antibody
Goat Polyclonal
5 References
200-101-200
200-101-C45S
200-101-C45
50 mg
25 µL
100 µg
Lyophilized
Liquid (sterile filtered)
Liquid (sterile filtered)
WB, ELISA, Biochemical Assay, IP, Other
GST-Tag
Goat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-GST (GOAT) Antibody - 200-101-C45
goat anti-GST antibody, GST, Glutathione-S-Transferase
Goat
Polyclonal
IgG
Target Details
GST-Tag
Native Protein
Glutathione-S-Transferase [Schistosoma japonicum]
Anti-GST is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Application Details
ELISA, WB
Biochemical Assay, IP, Other
- View References
Anti-GST antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Glutathione-S-Transferase [Schistosoma japonicum] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.
Formulation
1.0 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store GST antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
GST antibody recognizes the GST tag. The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial, and microsomal (now designated as MAPEG) proteins. GSTs are present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function.] The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions. Anti-GST antibody is ideal for investigators involved in epitope research.
Koivunen, J et al. (2021). Integrin α11β1 is a receptor for collagen XIII. Cell and Tissue Research
Applications
SPR-Based Analyses
Kubota, M et al. (2021). Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction. Scientific Reports
Applications
WB, IB, PCA
Bolch, SN et al. (2016). A Splice Variant of Bardet-Biedl Syndrome 5 (BBS5) Protein that Is Selectively Expressed in Retina. PloS One
Applications
IP, Co-IP
Machida et al. (2015). Identification of stroke-associated-antigens via screening of recombinant proteins from the human expression cDNA library (SEREX). Journal of Translational Medicine
Applications
E, EIA; SDS, 2D-PAGE; WB, IB, PCA
Smith, TS et al. (2013). Light-dependent phosphorylation of Bardet-Biedl syndrome 5 in photoreceptor cells modulates its interaction with arrestin1. Cellular and Molecular Life Sciences : Cmls
Applications
WB, IB, PCA; IP, Co-IP
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.