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GluR1 phospho S845 Antibody
Rabbit Polyclonal
612-401-C83
150 µL
Liquid
WB, IHC
Mouse, Rat
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-GluR1 pS845 (RABBIT) Antibody - 612-401-C83
Glutamate receptor 1, GluR1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1, Glutamate receptor ionotropic, AMPA 1
Rabbit
Polyclonal
IgG
Target Details
Gria1 - View All Gria1 Products
Mouse, Rat
Phosphorylation
Conjugated Peptide
Anti-GluR1 pS845 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845.
Anti-GluR1 pS845 Antibody is directed against GluR1 protein phosphorylated at S845. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Minimal reactivity occurs against non-phosphorylated GluR1. Reactivity is completely eliminated by treatment with lambda-Phosphatase. Reactivity against GluR1 occurs from rat sources. However, reactivity is expected against the following species based on 100% sequence homology: human, mouse, and non-human primates.
P19490 - UniProtKB
NP_113796.1 - NCBI Protein
Application Details
IHC, WB
Anti-GluR1 pS845 antibody is tested for use in IHC and Western Blotting and suitable for IF and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 100 kDa in size corresponding to GluR1 protein phosphorylated at Ser 845 in Western Blots of rat brain extracts.
Formulation
Titrated value sufficient to run approximately 10 mini blots.
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-GluR1 pS845 antibody recognizes phosphorylated GluR1. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR. In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory. Anti-GluR1 pS845 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
