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GFP Antibody Biotin Conjugated
Rabbit Polyclonal
19 References
600-406-215
100 µg
Lyophilized
WB, ELISA, IHC, IF, Other, Purification
GFP, eGFP, rGFP
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-GFP (RABBIT) Antibody Biotin Conjugated - 600-406-215
rabbit anti-GFP antibody biotin conjugation, biotin conjugated rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Rabbit
Biotin
Polyclonal
IgG
Target Details
GFP, eGFP, rGFP
Recombinant Protein
Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Anti-GFP was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse and Rat Serum Proteins.
P42212 - UniProtKB
Application Details
ELISA, WB
IF, IHC, Other, Purification
- View References
Biotin Conjugated GFP Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store Anti-GFP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
GFP Antibody is produced from Green fluorescent protein which is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Djaja N et al. (2023). Protocol for single-molecule pull-down of fluorescently tagged oligomers from cell lysates. STAR Protoc.
Applications
SiMPull
Husbands AY et al. (2023). The START domain potentiates HD-ZIPIII transcriptional activity. Plant Cell.
Applications
WB, IB, PCA
Djaja NA et al. (2023). Nucleation and dissolution mechanism underlying amyotrophic lateral sclerosis/frontotemporal lobar dementia-linked fused in sarcoma condensates. iScience.
Applications
SiMPull
Hartooni N et al. (2022). Single-molecule analysis of specificity and multivalency in binding of short linear substrate motifs to the APC/C. Nat Commun.
Applications
IF, Confocal Microscopy
Rhine K et al. (2022). Single molecule probing of disordered RNA binding proteins. STAR Protoc.
Applications
IF, Confocal Microscopy
Hannaford MR et al. (2022). Pericentrin interacts with Kinesin-1 to drive centriole motility. J Cell Biol.
Applications
Biolayer Interferometry Competition Assay
Liu R et al. (2021). A binding protein regulates myosin-7a dimerization and actin bundle assembly. Nat Commun.
Applications
Other
Aryal SP et al. (2021). Single-molecule studies of membrane receptors from brain region specific nanovesicles. Bio Protoc.
Applications
Undefined
Niaki AG et al. (2020). Loss of dynamic RNA interaction and aberrant phase separation induced by two distinct types of ALS/FTD-linked FUS mutations. Mol Cell.
Applications
IF, Confocal Microscopy
Priyanka Kothari. (2020). DECIPHERING THE BIOCHEMICAL INTERACTIONS OF THE MECHANORESPONSIVE CONTRACTILITY CONTROLLER Johns Hopkins University
Applications
SiMPull
Kothari P et al. (2019). Contractility kits promote assembly of the mechanoresponsive cytoskeletal network. J Cell Sci.
Applications
IF, Confocal Microscopy
Wen HJ et al. (2019). Myeloid cell-derived HB-EGF drives tissue recovery after pancreatitis. Cell Mol Gastroenterol.
Applications
IHC, ICC, Histology
Zhang H et al. (2019). MLL1 inhibition and vitamin D signaling cooperate to facilitate the expanded pluripotency state. Cell Rep.
Applications
IHC, ICC, Histology
Lee, J et al. (2019). A Myt1 family transcription factor defines neuronal fate by repressing non-neuronal genes. ELife
Applications
SiMPull
Wang et al. (2015). Dynamic profiling of double-stranded RNA binding proteins. Nucleic Acids Research
Applications
SiMPull
Han, KY et al. (2013). Understanding the photophysics of the spinach-DFHBI RNA aptamer-fluorogen complex to improve live-cell RNA imaging. Journal of the American Chemical Society
Applications
Ab Immobilization
Jain, A et al. (2011). Probing cellular protein complexes using single-molecule pull-down. Nature
Applications
WB, IB, PCA
Zanger RC et al. (2009). ProMAT Calibrator: a tool for reducing experimental bias in antibody microarrays. J Proteome Res.
Applications
EIA, ELISA
Graf ER et al. (2004). Neurexins induce differentiation of GABA and glutamate postsynaptic specializations via neuroligins. Cell.
Applications
Ab Immobilization
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
