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GAPDH Antibody
Rabbit Polyclonal
2 References
600-401-GV0S
600-401-GV0
25 µL
100 µg
Liquid (sterile filtered)
Liquid (sterile filtered)
WB, ELISA
Human, Mouse
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (RABBIT) Antibody - 600-401-GV0
rabbit anti-GAPDH antibody, rabbit anti-Glyceraldehyde-3-phosphate dehydrogenase antibody, GAPDH Loading Control Antibody, Anti-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD, Peptidyl-cysteine S-nitrosylase GAPDH
Rabbit
Polyclonal
IgG
Target Details
GAPDH - View All GAPDH Products
Human, Mouse
Conjugated Peptide
GAPDH Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH).
Anti-GAPDH Antibody is directed against human GAPDH protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest that this antibody would react with GAPDH from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
Application Details
ELISA, WB
Anti-GAPDH Antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~36 kDa in size corresponding to GAPDH by western blotting in the appropriate cell lysate or extract. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control marker. This Anti-GAPDH Antibody product is a new product replacing p/n 600-401-A33.
Formulation
1.17 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store Anti-GAPDH antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
GAPDH loading control antibody is ideal for Western Blotting, ELISA, IHC and IF Microscopy. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control antibody in immunoblots. A loading control antibody is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease. Anti-GAPDH antibody is ideal for investigators involved in apoptosis, cancer, DNA damage and repair and neuroscience.
Quiñones JL. et al. (2020). Oxidative DNA-protein crosslinks formed in mammalian cells by abasic site lyases involved in DNA repair. DNA Repair (Amst)
Applications
WB, IB, PCA
Matthias Nahrendorf et al. (2008). Activatable magnetic resonance imaging agent reports myeloperoxidase activity in healing infarcts and noninvasively detects the antiinflammatory effects of atorvastatin on ischemia-reperfusion injury. Circulation.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
