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GABA(A) Receptor beta 3 phospho S408/phospho S409 Antibody
Rabbit Polyclonal
2 References
612-401-D51
100 µL
Liquid
WB
Mouse, Rat
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-GABA(A) Receptor beta 3 pS408/pS409 (RABBIT) Antibody - 612-401-D51
Gamma-aminobutyric acid receptor subunit beta-3, GABA(A) receptor subunit beta-3, Gabrb3, GABA(A) receptor subunit ß-3
Rabbit
Polyclonal
IgG
Target Details
Gabrb3 - View All Gabrb3 Products
Mouse, Rat
Phosphorylation
Conjugated Peptide
Anti-GABA(A) Receptor beta 3 pS408/pS409 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser408/Ser409 of rat GABAA.
Anti-GABA(A) Receptor beta 3 pS408/pS409 Antibody is directed against rat GABA(A) Receptor beta 3. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Immunolabeling of the GABAA band is completely blocked by the phospho-peptide used as antigen but not by the corresponding dephospho-peptide. Reactivity is expected from the following species based on 100% sequence homology: bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish.
P63079.1 - UniProtKB
NP_058761.1 - NCBI Protein
Application Details
WB
Anti-GABA(A) Receptor beta 3 pS408/pS409 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 53 kDa in size corresponding to GABA(A) receptor beta 3 subunit phosphorylated at Ser408/Ser409 in the appropriate cell lysate or extract.
Formulation
Titrated value sufficient to run approximately 10 mini blots.
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. For extended storage, aliquot contents and freeze at -80° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-GABA(A) Receptor beta 3 pS408/pS409 Antibody detects GABA(A) Receptor beta 3. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma- subunit is required for benzodiazepine modulation. Phosphorylation of serine 408 and serine 409 within the beta3 subunit have been shown to be critical for the functional modulation of beta3 containing recombinant receptors. GABA(A) Receptor beta 3 pS408/pS409 antibody is ideal for investigators involved in Neuroscience.
Garcia JD et al. (2023). Distinct mechanisms drive sequential internalization and degradation of GABAARs during global ischemia and reperfusion injury. iScience.
Applications
WB, IB, PCA
Pribiag H et al. (2013). TNF-α downregulates inhibitory neurotransmission through protein phosphatase 1-dependent trafficking of GABAA receptors. J Neurosci.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
