Anti-Fructose-6-Phosphate Kinase Antibody - Immunofluorescence Microscopy

IgG Fraction of Anti-Fructose-6-Phosphate Kinase Antibody - Western Blot

Datasheet

Fructose-6-Phosphate Kinase Antibody

Name: Fructose-6-Phosphate Kinase Antibody
Brand: Rockland Immunochemicals
Availability: InStock
Rating: 5 (2 reviews)

Goat Polyclonal

2 References

Item No. 200-1156

Size: 50 mg

Physical State: Lyophilized

Applications: WB, ELISA, IF, Multiplex

Reactivity: Rabbit

Host Species: Goat

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50 mg $0.00

25 µL $125.00

100 µg $475.00

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Specifications for Fructose-6-Phosphate Kinase Antibody

Product Details

Description: Anti-Fructose-6-Phosphate Kinase (GOAT) Antibody (BULK ORDER) - 200-1156

Synonyms: goat anti-Fructose-6-Phosphate Kinase Antibody, 6 Phosphofructokinase Muscle Type antibody, GSD7 antibody, MGC8699 antibody, PFKA antibody, PFKL antibody, PFKM antibody, PFKP antibody, PFKX antibody, Phosphofructo 1 Kinase Isozyme A antibody, Phosphofructokinase 1 antibody

Host Species: Goat

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: PFKM - View All PFKM Products

Reactivity: Rabbit

Immunogen Type: Native Protein

Immunogen: Full length native Fructose-6-Phosphate Kinase purified from rabbit muscle

Purity/Specificity: Anti-FRUCTOSE-6-PHOSPHATE KINASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources is expected based on high degrees of sequence homology for muscle derived F6PK. Partial reactivity may occur against F6PK isolated from liver.

Database Links:

P00511 - UniProtKB

125128 - NCBI Protein

100345647 - Gene ID

Application Details

Tested Applications: ELISA, IF, WB

Suggested Applications: Multiplex - View References

Application Note: FRUCTOSE-6-PHOSPHATE KINASE antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.

Formulation

Concentration: 10.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Reconstitution Volume: 5.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Fructose-6-Phosphate Kinase -2 (F6PK) also known as Phosphofructokinase (PFK) catalyzes the conversion of ATP + D-fructose 6-phosphate to ADP + D-fructose 1,6-bisphosphate and therefore is a key enzyme in the control of glycolysis and carbohydrate degradation. This is a unidirectional and rate-limiting step in glycolysis. Allosteric kinetics control activation by ADP, AMP, or fructose bisphosphate and inhibition by ATP or citrate. The enzyme exists as a homotetramer.

References (2)

Cheng Z et al. (2021). Normobaric oxygen therapy attenuates hyperglycolysis in ischemic stroke. Neural Regan Res.

Applications

WB, IB, PCA

PubMed

Vallejo J, Hardin CD. (2004). Metabolic organization in vascular smooth muscle: distribution and localization of caveolin-1 and phosphofructokinase. Am J Physiol Cell Physiol.

Applications

IF, Confocal Microscopy; Multiplex Assay

PubMed

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.