Datasheet

Fructose-6-Phosphate Kinase Antibody

Name: Fructose-6-Phosphate Kinase Antibody
Brand: Rockland Immunochemicals
Price: 1225.0 USD
Availability: InStock
Rating: 5 (2 reviews)

Goat Polyclonal

2 References

Item No. 100-1156

Size: 2 mL

Physical State: Lyophilized

Applications: WB

Reactivity: Rabbit

Host Species: Goat

$1,225.00 /Per Item

In stock

Ships next business day

Add To Cart

Add to List

Bulk Orders

Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Specifications for Fructose-6-Phosphate Kinase Antibody

Product Details

Description: Anti-Fructose-6-Phosphate Kinase (Rabbit Muscle) (GOAT) Antibody - 100-1156

Synonyms: goat anti-Fructose-6-Phosphate Kinase Antibody, 6 Phosphofructokinase Muscle Type antibody, GSD7 antibody, MGC8699 antibody, PFKA antibody, PFKL antibody, PFKM antibody, PFKP antibody, PFKX antibody, Phosphofructo 1 Kinase Isozyme A antibody, Phosphofructokinase 1 antibody

Host Species: Goat

Clonality: Polyclonal

Format: Antiserum

Target Details

Gene Name: PFKM - View All PFKM Products

Reactivity: Rabbit

Immunogen Type: Native Protein

Immunogen: Fructose-6-Phosphate Kinase [Rabbit Muscle]

Purity/Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources may occur but has not been specifically determined.

Database Links:

P00511 - UniProtKB

125128 - NCBI Protein

100345647 - Gene ID

Application Details

Suggested Applications: WB - View References

Application Note: This antibody suitable for use in ELISA, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.

Formulation

Concentration: 90 mg/mL by Refractometry

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Fructose-6-Phosphate Kinase -2 (F6PK) also known as Phosphofructokinase (PFK) catalyzes the conversion of ATP + D-fructose 6-phosphate to ADP + D-fructose 1,6-bisphosphate and therefore is a key enzyme in the control of glycolysis and carbohydrate degradation. This is a unidirectional and rate-limiting step in glycolysis. Allosteric kinetics control activation by ADP, AMP, or fructose bisphosphate and inhibition by ATP or citrate. The enzyme exists as a homotetramer.

References (2)

Heinisch, J.J. et al. (2020). Investigation of Heterologously Expressed Glucose-6-Phosphate Dehydrogenase Genes in a Yeast zwf1 Deletion Microorganisms

Applications

WB, IB, PCA

PubMed

Nakamura et al. (2010). Molecular complex of three testis-specific isozymes associated with the mouse sperm fibrous sheath: hexokinase 1, phosphofructokinase M, and glutathione S-transferase mu class 5. Biol Reprod.

Applications

WB, IB, PCA

PubMed

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.