Immunofluorescence of Anti-Fibronectin Antibody

Immunohistochemistry of Anti-Fibronectin Antibody

Western Blot of Anti-Fibronectin Antibody

Datasheet SDS

Fibronectin Antibody

Name: Fibronectin Antibody
Brand: Rockland Immunochemicals
Price: 949.0 USD
Availability: InStock
Rating: 5 (5 reviews)

Rabbit Polyclonal

5 References

Item No. 600-401-117-0.1

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: WB, IHC, IF

Reactivity: Human

Host Species: Rabbit

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500 µg $949.00

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Specifications for Fibronectin Antibody

Product Details

Description: Anti-Fibronectin (Human) (RABBIT) Antibody - 600-401-117-0.1

Synonyms: rabbit anti-Fibronectin antibody, FN1, FN, Cold-insoluble globulin, CIG, Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: FN1 - View All FN1 Products

Reactivity: Human

Immunogen Type: Native Protein

Immunogen: Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE.

Purity/Specificity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.

Database Links:

P02751 - UniProtKB

AAA53376.1 - NCBI Protein

2335 - Gene ID

Application Details

Suggested Applications: IF, IHC, WB - View References

Application Note: Anti-Fibronectin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Rockland's anti-Fibronectin detects intact fibronectin (Invitrogen, Cat. No. 33016-015) by western blot after digestion by Matrix Metalloproteinase-3 (MMP-3) overnight at 37° C. Separation was performed using a 4-12% Tris-Glycine gel. Under these conditions a sizeable, dark band at ~220 kDa representing the undigested fibronectin, as well as many, smaller bands representing the variably sized fragments resulting from fibronectin digestion by MMP-3 were noted. For immunohistochemistry paraffin embedded tissue preparation is recommended.

Formulation

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Human fibronectin has a molecular weight of 450,000 daltons when purified in an intact form from plasma. Fibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form, and is synthesized by many mesenchymal cells, for the extracellular matrix form. It is composed of two similar, but not identical protein chains, which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason, detection by immunoblot (western) may show considerable variability in the observed apparent molecular weights, which is predicated on the source of the fibronectin, and to what degree proteolysis has occurred. Bands approximately 225 kDa should be observed after SDS-PAGE when reducing and denaturing conditions are used.

References (5)

Schulz MC et al. (2019). Epithelial-fibroblast cross talk aggravates the impact of the nephrotoxin ochratoxin A. Biochem Biophys Acta Mol Cell Res.

Applications

WB, IB, PCA

PubMed

Parween et al. (2016). Intra-islet lesions and lobular variations in β-cell mass expansion in ob/ob mice revealed by 3D imaging of intact pancreas. Scientific Reports

Applications

IHC, ICC, Histology

PubMed

Teichmann J et al. (2012). The control of endothelial cell adhesion and migration by shear stress and matrix-substrate anchorage. Biomaterials.

Applications

IF, Confocal Microscopy

PubMed

Hansen U et al. (2006). The anchorless adhesin Eap (extracellular adherence protein) from Staphylococcus aureus selectively recognizes extracellular matrix aggregates but binds promiscuously to monomeric matrix macromolecules. Matrix Biol.

Applications

WB, IB, PCA

PubMed

May M et al. (2006). Identification of Fibronectin-Binding Proteins in Mycoplasma gallisepticum Strain R. Infect Immun.

Applications

WB, IB, PCA

PubMed

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Heat-induced Antigen Retrieval Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Immunohistochemistry (IHC) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Protease-induced Antigen Retrieval Protocol

Staining Paraffin Sections by PAP Procedure

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.