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Ferritin Antibody
Rabbit Polyclonal
10 References
200-401-090
200-401-090S
200-401-090-0100
50 mg
25 µL
100 µg
Lyophilized
Liquid (sterile filtered)
Lyophilized
WB, ELISA, IF, FC, LFA, Multiplex
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Ferritin (Human Spleen) (RABBIT) Antibody (BULK ORDER) - 200-401-090
rabbit anti-Ferritin Antibody, FTHL6, Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH
Rabbit
Polyclonal
IgG
Target Details
FTH1 - View All FTH1 Products
Human
Native Protein
Ferritin [Human Spleen]
Anti-Ferritin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen]. Cross reactivity against Ferritin from other tissues and species may occur but have not been specifically determined.
Application Details
ELISA, WB
FC, IF, LFA, Multiplex
- View References
Anti-Ferritin has been tested in ELISA and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Formulation
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
5.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Ferritin stores iron in a soluble, non-toxic, readily available form. It is important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Ferritin also plays a role in delivery of iron to cells and mediates iron uptake in capsule cells of the developing kidney.
Yu, F et al. (2022). Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis. Cell Discovery
Applications
IF, Confocal Microscopy
Ohta K et al. (2021). Human Parainfluenza Virus Type 2 V Protein Modulates Iron Homeostasis. J Virol.
Applications
Undefined
Guo J et al. (2021). A combined model of human iPSC‐derived liver organoids and hepatocytes reveals ferroptosis in DGUOK mutant mtDNA depletion syndrome. Adv Sci (Weinh).
Applications
IF, Confocal Microscopy
Yap BK et al. (2018). Potential point-of-care microfluidic devices to diagnose iron deficiency anemia. Sensors (Basel).
Applications
Lateral Flow (LFA)
Kimura et al. (2017). Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy. The EMBO Journal
Applications
IF, Confocal Microscopy; WB, IB, PCA
Goodwin et al. (2017). Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9. Cell Reports
Applications
FC, FACS, FLOW; IF, Confocal Microscopy; Multiplex Assay
Mai et al. (2017). Salinomycin kills cancer stem cells by sequestering iron in lysosomes. Nature Chemistry
Applications
IF, Confocal Microscopy
Mancias et al. (2015). Ferritinophagy via NCOA4 is required for erythropoiesis and is regulated by iron dependent HERC2-mediated proteolysis. Elife
Applications
IF, Confocal Microscopy
Mancias JD et al. (2014). Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy. Nature.
Applications
IF, Confocal Microscopy; Multiplex Assay
Dowdle WE et al. (2014). Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo. Nat Cell Biol.
Applications
IF, Confocal Microscopy
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
