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Datasheet

Fab Biotin Antibody Alkaline Phosphatase Conjugated

Goat Polyclonal

800-105-098
1 mg
Liquid (sterile filtered)
ELISA
Biotin
Goat
$609.00 /Per Item
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

Fab Anti-Biotin (GOAT) Antibody Alkaline Phosphatase Conjugated - 800-105-098
Goat Fab Anti-Biotin Antibody Alkaline Phosphatase Conjugation, Goat Fab Anti-Biotin HRP Conjugated Antibody
Goat
Alkaline Phosphatase (AP)
Polyclonal
IgG Fab

Target Details

Biotin
Other
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Fab Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using biotin coupled to sepharose beads followed by papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum.  No reaction was observed against anti-papain or anti-Goat IgG F(c).

Application Details

ELISA
Fab Anti-Biotin Antibody Alkaline Phosphatase Conjugated has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity should be optimized by the end user.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Shipping & Handling

Wet Ice
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Expiration date is one (1) year from date of receipt.
Fab Biotin antibody recognizes Biotin. Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.