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F4/80 Allophycocyanin Antibody
Rat Monoclonal BM8.1 IgG2a kappa
200-526-L55
100 µg
Liquid (sterile filtered)
FC
Mouse
Rat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-F4/80 (RAT) Allophycocyanin Conjugated Monoclonal Antibody - 200-526-L55
EMR1, Ly71, EGF-TM7, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor, Gpf480, Adgre1
Rat
Allophycocyanine (APC)
Monoclonal
IgG2a
Target Details
Adgre1 - View All Adgre1 Products
Mouse
Anti-F4/80 Antibody (Monoclonal) was produced by repeated immunizations with F4/80 antigen.
Allophycocyanin conjugated F4/80 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse F4/80. Cross reactivity with F4/80 from other sources has not been tested. Anti-F4/80 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.
Application Details
FC
Anti-F4/80 is tested for Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
Formulation
0.2mg/ml by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.09% (w/v) Sodium Azide
0.1% Gelatin
Shipping & Handling
Wet Ice
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Expiration date is six (6) months from date of receipt.
The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
