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Datasheet

F-Box Only Protein 9 Antibody

Rabbit Polyclonal

600-401-918
100 µg
Liquid (sterile filtered)
WB, ELISA
Human
Rabbit
$499.00 /Per Item
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

Anti-F-Box Only Protein 9 (FBOX9) (RABBIT) Antibody - 600-401-918
rabbit anti-F-Box Only Protein 9 antibody, rabbit anti-FBXO9 antibody, FBX9, VCIA1, Cross-immune reaction antigen 1 antibody, Renal carcinoma antigen NY-REN-57 antibody
Rabbit
Polyclonal
IgG

Target Details

FBXO9  - View All FBXO9 Products
Human
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-447 of human FBOX9 protein.
This affinity-purified antibody is directed against human FBOX9 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross reactivity with FBOX9 protein from human, dog, rat and chimpanzee based on 100% homology with the immunizing sequence.  Expect partial reactivity with homologues from zebrafish, bovine and mouse (94% homology) as well as chicken (88%) and Xenopus laevis (82%).  Reactivity is expected against all known isoforms of this protein, as the immunogen sequence is common to their carboxy-terminal ends.  Reactivity against homologues from other sources is not known.
Q9UK97 - UniProtKB
53692184 - NCBI Protein
26268 - Gene ID

Application Details

ELISA, WB
This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user.  Although the predicted MW of FBOX9 isoform 1 is 52 kDa, antibody reactivity against MCF7 whole cell lysates shows a predominant band at 100 kDa.  This band is believed to be FBOX9 and the higher apparent MW may be due to its association with other proteins.  Isoforms 1, 2 and 3 have reported molecular weights of 52.3, 51.1 and 47.3 kDa, respectively.

Formulation

1.53 mg/mL
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
F-box only protein 9 (also called FBOX9 and Fbp9) is a member of the F-box protein family, which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Anti-F-Box 9 Antibody is useful for researchers interested in Multiple Myeloma and Epilepsy, as well as the immune system and Ubiquitin pathway research.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.