Datasheet

EZH2 Antibody

Name: EZH2 Antibody
Brand: Rockland Immunochemicals
Availability: OutOfStock

Mouse Monoclonal IgG1

Item No. 200-301-W46

Size: 50 µg

Physical State: Liquid (sterile filtered)

Applications: ChIP

Reactivity: Human

Host Species: Mouse

This product is discontinued View alternative product

Specifications for EZH2 Antibody

Product Details

Description: Anti-EZH2 (MOUSE) Monoclonal Antibody - 200-301-W46

Synonyms: Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, KMT6

Host Species: Mouse

Clonality: Monoclonal

Format: IgG1

Target Details

Gene Name: EZH2 - View All EZH2 Products

Reactivity: Human

Immunogen Type: Conjugated Peptide

Immunogen: Anti-EZH2 Antibody was produced in mice by repeated immunizations containing an amino acid sequence from the internal region of the human EZH2 protein.

Purity/Specificity: Anti-EZH2 Antibody was purified by Protein G chromatography. Cross-reactivity with EZH2 from other species has not been determined.

Database Links:

Q15910 - UniProtKB

NP_001190176.1 - NCBI Protein

2146 - Gene ID

Application Details

Tested Applications: ChIP

Application Note: Anti-EZH2 Antibody is tested for Chromatin Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.

Formulation

Concentration: 1 mg/ml by UV absorbance at 280 nm

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Preservative: 0.05% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

EZH2 is a histone-lysine methyltransferase which methylates ‘Lys-9’ and ‘Lys-27’ of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and internal nervous systems. Over-expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer. Anti-EZH2 Antibody is ideal for research in Epigenetics, Gene Expression, Neuroscience and Cancer.

Related Protocols

Adherent Cell Lysis Protocol

Bifunctional Cross-Linking Protocol

Chromatin Immunoprecipitation (ChIP) Protocol

Immunoprecipitation (IP) Protocol

IP-WB with TrueBlot® Protocol

Nucleosome ChIP Protocol

Re-ChIP Protocol

Suspension Cultured Cell Lysis Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.