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Erpd/Arp37 Antibody
Rabbit Polyclonal
1 References
200-401-C09S
200-401-C09
25 µL
100 µg
Liquid (sterile filtered)
Lyophilized
WB, ELISA
Borrelia burgdorferi
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-ErpD/Arp37 (RABBIT) Antibody - 200-401-C09
Rabbit anti-ErpD Antibody, rabbit anti-Arp37 Antibody, rabbit anti-Arp37/ErpD Antibody, Arp37, Borrelia burgdorferi ErpD
Rabbit
Polyclonal
IgG
Target Details
BB_F01 - View All BB_F01 Products
Borrelia burgdorferi
Recombinant Protein
MBP-fusion protein corresponding to Borrelia burgdorferi ErpD/Arp37 protein.
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. It is directed against, and shows specific reactivity for, Borrelia burgdorferi ErpD protein. Reactivity with ErpD/Arp37 protein from other sources has not been determined.
Application Details
ELISA, WB
Anti-ErpD antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect bands at ~37.5 kDa and ~65 kDa in size corresponding to ErpD/Arp37 by Western blotting in the appropriate cell lysate or extract.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
100 µL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
This product is antibody made against ErpD (ospE/F-Related Protein D), also known as Arp37, from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. Erp proteins from Borrelia burgdorferi are postulated to be lipoproteins, based on their predicted amino acid sequences. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. Several studies have demonstrated that infected humans and animals produce antibodies directed against Erp proteins within the first 2-4 weeks of infection, indicative of Erp synthesis during the initial stages of vertebrate infection. It is postulated that surface-exposed Erp proteins could facilitate interactions with host tissues during the establishment of vertebrate infection.
Lone A et al. (2020). The Borrelia burgdorferi VlsE Lipoprotein Prevents Antibody Binding to an Arthritis-Related Surface Antigen. Cell Rep.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
