Western Blot of Monoclonal Anti-ERK2 Antibody DyLight™680 Conjugated

Datasheet

ERK2 Internal Antibody DyLight™680

Name: ERK2 Internal Antibody DyLight™680
Brand: Rockland Immunochemicals
Price: 125.0 USD
Availability: OutOfStock

Mouse Monoclonal 3H9.A1.A8

Item No. 200-344-GQ1

Size: 100 µg

Physical State: Lyophilized

Applications: WB

Reactivity: Human, Mouse, Rat

Host Species: Mouse

$495.00 /Per Item

25 µL $125.00

100 µg $495.00

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Specifications for ERK2 Internal Antibody DyLight™680

Product Details

Description: Anti-ERK2 (MOUSE) Monoclonal Antibody DyLight™680 Conjugated - 200-344-GQ1

Synonyms: mouse anti-ERK2 antibody DyLight™680 conjugation, DyLight™680 conjugated mouse anti-ERK2 antibody, MAPK1, ERK, ERK-2, ERK2, MAPK2, P42MAPK, PRKM1, PRKM2, DyLight™680, ERK-2 Antibody

Host Species: Mouse

Conjugate: DyLight™ 680

Clonality: Monoclonal

Format: IgG

Target Details

Gene Name: MAPK1 - View All MAPK1 Products

Reactivity: Human, Mouse, Rat

Immunogen Type: Conjugated Peptide

Immunogen: Anti-ERK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.

Purity/Specificity: This DyLight™680 conjugated protein A purified mouse monoclonal antibody reacts specifically with human ERK2. Anti-ERK2 is purified from tissue culture supernatant by protein A purification. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK1 isoform.

Database Links:

P28482 - UniProtKB

Application Details

Tested Applications: WB

Application Note: Anti-ERK 2 (MOUSE) DyLight™680 conjugated antibody is suitable for use in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 41 kDa.

Formulation

Concentration: 1mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 100 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

ERK2 antibodies detect the ERK2 isoform. Mitogen activated protein kinase 1, also known as MAPK1, ERK, or ERK2, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. ERK1 and ERK2 are activated by MEK1 and MEK2 in the B-raf signaling pathway resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Human ERK1 and ERK2 are 84% identical in sequence and share common functionality in cells. Anti-ERK2 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.