Datasheet

EndoG Antibody [7G1C10]

Name: EndoG Antibody [7G1C10]
Brand: Rockland Immunochemicals
Price: 485.0 USD
Availability: OutOfStock

Mouse Monoclonal [7G1C10] IgG1

Item No. 200-301-BA8

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Host Species: Mouse

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Specifications for EndoG Antibody [7G1C10]

Product Details

Description: Anti-EndoG (MOUSE) Antibody - 200-301-BA8

Synonyms: EndoG Antibody [7G1C10] , Endo G

Host Species: Mouse

Clonality: Monoclonal

Format: IgG1

Target Details

Gene Name: ENDOG - View All ENDOG Products

Reactivity: Human, Mouse, Rat

Immunogen Type: Recombinant Protein

Immunogen: Anti-EndoG antibody was produced in mice by repeated immunizations with recombinant protein corresponding to amino acids 51 - 140 of human EndoG.

Purity/Specificity: Anti-EndoG Monoclonal Antibody was Protein A purified. Cross reactivity with EndoG [7G1C10] from other sources has not been determined.

Database Links:

Q14249 - UniProtKB

NP_004426.2 - NCBI Protein

2021 - Gene ID

Application Details

Tested Applications: ELISA, WB

Application Note: Anti-EndoG Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 33 kDa in Western Blots of specific cell lysates and tissues. Validated in human samples. All other applications and species not yet tested.

Formulation

Concentration: 1 mg/mL by UV absorbance at 280 nm

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Preservative: 0.02% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.