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Damage Related Autophagy Modulator Antibody
Rabbit Polyclonal
5 References
600-401-A70
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC, IF
Human, Mouse, Rat
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Damage Related Autophagy Modulator (RABBIT) Antibody - 600-401-A70
DRAM antibody, FLJ11259 antibody, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator, DRAM1
Rabbit
Polyclonal
IgG
Target Details
DRAM1 - View All DRAM1 Products
Human, Mouse, Rat
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human DRAM protein.
This affinity purified antibody is directed against human DRAM protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with DRAM from human, mouse and rat sources. Reactivity with DRAM from other sources has not been determined.
Application Details
ELISA, IF, IHC, WB
This affinity purified antibody has been tested for use in ELISA, immunofluorescence, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~33 kDa in size corresponding to DRAM by western blotting in the appropriate cell lysate or extract. K562 cell lysate can be used as positive control.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes together promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.
Vega-Naredo I et al. (2021). Analysis of Proapoptotic Protein Trafficking to and from Mitochondria. Methods Mol Biol.
Applications
Undefined
Magalhaes-Novais S et al. (2020). Cell quality control mechanisms maintain stemness and differentiation potential of P19 embryonic carcinoma cells. Autophagy.
Applications
WB, IB, PCA
Vega-Naredo I et al. (2015). Analysis of pro-apoptotic protein trafficking to and from mitochondria. Methods Mol Biol.
Applications
IHC, ICC, Histology; WB, IB, PCA
Vega-Naredo I et al. (2012). Melatonin modulates autophagy through a redox‐mediated action in female Syrian hamster Harderian gland controlling cell types and gland activity. J Pineal Res.
Applications
WB, IB, PCA
Vega-Naredo I et al. (2009). Sexual dimorphism of autophagy in Syrian hamster Harderian gland culminates in a holocrine secretion in female glands. Autophagy.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
