Cytokeratin Peptide 18 Antibody
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Datasheet

Cytokeratin Peptide 18 Antibody

Mouse Monoclonal CY-90 IgG1

200-301-P33
100 µg
Lyophilized
WB, IHC, IF
Human, Rat
Mouse
$485.00 /Per Item
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Product Details

Anti-Cytokeratin Peptide 18 (MOUSE) Antibody - 200-301-P33
K18, Keratin-18, CK-18, Cytokeratin-18, CYK18, Cell proliferation-inducing gene 46 protein
Mouse
Monoclonal
IgG1

Target Details

KRT18  - View All KRT18 Products
Human, Rat
Other
Cytokeratin Peptide 18 antibody was produced in mice by repeated immunizations with the human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.
Anti-Cytokeratin Peptide 18 antibody was purified from mouse ascites by protein A chromatography.  This product reacts with human and rat. Cross reactivity with Cytokeratin Peptide 18 from other sources has not been determined.
P05783 - UniProtKB
NP_000215.1 - NCBI Protein
3875 - Gene ID

Application Details

IF, IHC, WB
Anti-Cytokeratin Peptide 18 is tested for Immunohistochemistry-F, Immunohistochemistry-P, Immunofluorescence, and Western Blot. Expect a band approximately ~48 kDa corresponding to the appropriate cell lysate or extract. western blot tested in human. Specific conditions for reactivity should be optimized by the end user.

Formulation

100 µg/ml by UV absorbance at 280 nm
1.2% sodium acetate
0.01% (w/v) Sodium Azide
2mg BSA
1.0 mL
Neutral PBS

Shipping & Handling

Ambient
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis. This antibody is suitable for researchers interested in cancer research, cardiovascular diseases, and cytoskeletal signaling research.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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