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Cul1 Antibody
Rabbit Polyclonal
6 References
100-401-A01
100 µL
Liquid (sterile filtered)
WB, IHC, IP, Other
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Cul1 (C-terminal specific) (RABBIT) Antibody - 100-401-A01
rabbit anti-Cul1 Antibody, rabbit anti-Cullin1 antibody, MGC149834 antibody, MGC149835 antibody, CUL, Cullin
Rabbit
Polyclonal
Antiserum
Target Details
CUL1 - View All CUL1 Products
Human
Conjugated Peptide
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal near amino acids 750-776 of Human Cul1 coupled to KLH.
This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 1. Cross reactivity is expected against mouse Cul1 based on sequence homology.
Application Details
IHC, IP, WB
Other
- View References
Anti-Cul1 has been tested by immunohistochemistry. This antibody reacts with human Cul1 by immunohistochemistry, western blot, and immunoprecipitation. The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3). Do not IP in the presence of NP-40, but rather use 0.1% SDS. An 89.6 kDa band corresponding to human Cul1 is detected. Most cell lines expressing Cul1 can be used as a positive control. Researchers should determine optimal titers for other applications.
Formulation
85 mg/mL by Refractometry
None
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 1 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul1 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul1 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul1 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul1 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.
Islam S et al. (2021). FBXW8 regulates G1 and S phases of cell cycle progression by restricting β‐TrCP1 function. FEBS J.
Applications
Undefined
Islam S et al. (2021). β-TrCP1 facilitates cell cycle checkpoint activation, DNA repair and cell survival through ablation of β-TrCP2 in response to genotoxic stress. J Biol Chem.
Applications
WB, IB, PCA
Islam S et al. (2021). Co-operative binding of SKP1, Cullin1 and Cullin7 to FBXW8 results in Cullin1-SKP1-FBXW8-Cullin7 functional complex formation that monitors cellular function of β-TrCP1. Int J Biol Macromol.
Applications
Undefined
Islam S et al. (2021). Feedback‐regulated transcriptional repression of FBXO31 by c‐Myc triggers ovarian cancer tumorigenesis. Int J Cancer.
Applications
Undefined
Enchev RI et al. (2012). Structural basis for a reciprocal regulation between SCF and CSN. Cell Rep.
Applications
WB, IB, PCA
Duda DM et al. (2008). Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation. Cell.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.