Collagen Type VI Antibody
Rockland Anti-Collagen VI Antibody – Immunohistochemistry
Dot Blot of Collagen VI
Western Blot of Collagen VI
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Collagen Type VI Antibody

Rabbit Polyclonal

13 References
600-401-108S 600-401-108-0.1 600-401-108-0.5
25 µL 100 µg 500 µg
Liquid (sterile filtered) Liquid (sterile filtered) Liquid (sterile filtered)
WB, IHC, IF, Dot Blot, Multiplex
Human, Bovine
Rabbit
Collagen Type VI Antibody Biotin Conjugated Collagen Type VI Antibody Fluorescein Conjugated Collagen Type VI Antibody Peroxidase Conjugated
$125.00 /Per Item
$485.00 /Per Item
$949.00 /Per Item
25 µL $125.00
100 µg $485.00
500 µg $949.00
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Product Details

Anti-Collagen Type VI (RABBIT) Antibody - 600-401-108-0.1
rabbit anti-Collagen Type VI antibody, Collagen alpha-1 (VI) chain, Collagen VI antibody, Human mRNA for collagen VI alpha 1 C terminal globular domain antibody
Rabbit
Polyclonal
IgG

Target Details

COL6A1-COL6A3  - View All COL6A1-COL6A3 Products
Human, Bovine
Native Protein
Collagen Type VI from human and bovine placenta
Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.   This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens.  Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
P12111 - UniProtKB
AAH52575.1 - NCBI Protein
1291 - Gene ID

Application Details

Dot Blot, IHC
IF, WB, Multiplex  - View References
Anti-Collagen Type VI has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.

Formulation

1.19 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Expiration date is one (1) year from date of receipt.
Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
(). Spatial Transcriptomics Suggests That Alterations Occur in the Preneoplastic Breast Microenvironment of BRCA1/2 Mutation Carriers. Mol Cancer Res.
Applications
IHC, ICC, Histology
(). ROCK 1 and 2 affect the spatial architecture of 3D spheroids derived from human corneal stromal fibroblasts in different manners. Scientific Reports
Applications
IF, Confocal Microscopy
(). Comparison of the Drug-Induced Efficacies between Omidenepag Isopropyl, an EP2 Agonist and PGF2α toward TGF-β2-Modulated Human Trabecular Meshwork (HTM) Cells. Journal of Clinical Medicine
Applications
IF, Confocal Microscopy
(). Addition of ROCK Inhibitors Alleviates Prostaglandin-Induced Inhibition of Adipogenesis in 3T3L-1 Spheroids. Bioengineering (Basel).
Applications
IF, Confocal Microscopy
(). Myocardial Accumulations of Reg3A, Reg3γ and Oncostatin M are Associated With the Formation of Granulomata in Patients With Cardiac Sarcoidosis. Int J Mol Sci.
Applications
IF, Confocal Microscopy
(). Prostaglandin F2α agonist-induced suppression of 3T3-L1 cell adipogenesis affects spatial formation of extra-cellular matrix. Sci Rep
Applications
IF, Confocal Microscopy
(). HIF2A-LOX Pathway Promotes Fibrotic Tissue Remodeling in Thyroid-Associated Orbitopathy. Endocrinology.
Applications
IF, Confocal Microscopy
(). Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells. J Biol Chem.
Applications
IF, Confocal Microscopy
(). Early transcriptional alteration of histone deacetylases in a murine model of doxorubicin-induced cardiomyopathy. PLoS One.
Applications
IF, Confocal Microscopy
(). Pericellular colocalisation and interactive properties of type VI collagen and perlecan in the intervertebral disc. Eur Cell Mater.
Applications
IF, Confocal Microscopy; WB, IB, PCA; IHC, ICC, Histology
(). Development of myotendinous-like junctions that anchor cardiac valves requires fibromodulin and lumican. Dev Dyn.
Applications
IHC, ICC, Histology; Multiplex Assay
(). 3-D adipocyte differentiation and peri-adipocyte collagen turnover. Methods Enzymol.
Applications
IHC, ICC, Histology; IF, Confocal Microscopy
(). Three-dimensional morphology of the pericellular matrix of intervertebral disc cells in the rat. J Anat.
Applications
IHC, ICC, Histology

What were the immunogens for the collagen antibodies?

The immunogens for the collagen antibodies are the respective purified solubilized native collagen proteins (tropocollagens).

In what format are the collagen antibodies provided?

The collagen antibodies are provided in liquid format at a concentration of around 1 mg/mL (Absorbance 280 nm).

In what buffer are the collagen antibodies provided?

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide.

Are the antibodies specific to the reduced or non-reduced (native) proteins?

The collagen antibodies are specific to the native 3D epitopes found on the collagen native collagen protein. The collagen antibodies may have no reactivity to reduced collagen.

How was the reactivity of the antibodies tested? Was cross-reactivity to other collagens tested?

Each antibody was tested by immunoblot against each type of collagen (Type I – VI). Due to the close association of collagen proteins, cross-reactivity to other collagens may be observed. The optimal experimental conditions should be determined to minimize cross-reactivity in your application.

What are the recommended storage conditions for collagen antibodies?

The recommended storage temperature for collagen antibodies is -20°C. Please be sure to add glycerol 1:1 before freezing. We recommend making aliquots for best stability. The antibodies are stable for several weeks at 4°C.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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