Collagen Type IV Antibody
Immunofluorescence of Goat Anti-Collagen Type IV Antibody
Immunohistochemistry of Anti-Collagen Type IV Antibody
Immunohistochemistry of Goat Anti-Collagen Type IV Antibody
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Collagen Type IV Antibody

Goat Polyclonal

600-101-MN4
100 µg
Liquid (sterile filtered)
ELISA, IHC, IF
Mouse
Goat
$485.00 /Per Item
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Product Details

Anti-Collagen Type IV (GOAT) Antibody - 600-101-MN4
Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody, alpha unit Collagen IV Type IV Collagen, Col4, Collagen alpha-1(IV) chain
Goat
Polyclonal
IgG

Target Details

COL4A1  - View All COL4A1 Products
Mouse
Conjugated Peptide
Anti-Collagen IV antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 240-265 of mouse Collagen IV.
This affinity purified antibody is directed against mouse Collagen IV. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with mouse and 78.6% identity with human.
P02463 - UniProtKB
NP_034061.2 - NCBI Protein
12826 - Gene ID

Application Details

ELISA, IF, IHC
Anti-Collagen IV antibody has tested in ELISA, IF, and IHC. This antibody is suitable in western blot, expect a band at ~160kDa in western blot using appropriate tissues or lysates. Positive control used mouse placenta and mouse testes in immunohistochemistry, and NIH/3T3 in immunofluorescence.

Formulation

1.18 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagen Type IV proteins are integral components of basement membranes. This type of collagen is found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Anti-Collagen Type IV Antibody is ideal for researchers involved in extracellular matrix proteins and nephritis.

What were the immunogens for the collagen antibodies?

The immunogens for the collagen antibodies are the respective purified solubilized native collagen proteins (tropocollagens).

In what format are the collagen antibodies provided?

The collagen antibodies are provided in liquid format at a concentration of around 1 mg/mL (Absorbance 280 nm).

In what buffer are the collagen antibodies provided?

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide.

Are the antibodies specific to the reduced or non-reduced (native) proteins?

The collagen antibodies are specific to the native 3D epitopes found on the collagen native collagen protein. The collagen antibodies may have no reactivity to reduced collagen.

How was the reactivity of the antibodies tested? Was cross-reactivity to other collagens tested?

Each antibody was tested by immunoblot against each type of collagen (Type I – VI). Due to the close association of collagen proteins, cross-reactivity to other collagens may be observed. The optimal experimental conditions should be determined to minimize cross-reactivity in your application.

What are the recommended storage conditions for collagen antibodies?

The recommended storage temperature for collagen antibodies is -20°C. Please be sure to add glycerol 1:1 before freezing. We recommend making aliquots for best stability. The antibodies are stable for several weeks at 4°C.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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