Collagen Type III Antibody Peroxidase Conjugated
Immunohistochemistry of Anti-Collagen Type III Peroxidase Conjugated
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Datasheet

Collagen Type III Antibody Peroxidase Conjugated

Rabbit Polyclonal

1 References
600-403-105
50 µg
Lyophilized
IHC
Human, Bovine
Rabbit
Collagen Type III Antibody Collagen Type III Antibody Biotin Conjugated Collagen Type III Antibody Fluorescein Conjugated
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Product Details

Anti-Collagen Type III (RABBIT) Antibody Peroxidase Conjugated - 600-403-105
rabbit anti-Collagen Type III antibody peroxidase conjugation, HRP conjugated rabbit anti-Collagen Type III antibody, Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody, COL3A1, Collagen alpha-1 (III) chain
Rabbit
Peroxidase (HRP)
Polyclonal
IgG

Target Details

COL3A1  - View All COL3A1 Products
Human, Bovine
Native Protein
Collagen Type III from human and bovine placenta
This product has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
P02461 - UniProtKB
NP_000081.1 - NCBI Protein
1281 - Gene ID

Application Details

IHC  - View References
Anti-Collagen Type III Peroxidase Conjugated Antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.01% (w/v) Thimerosal
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
50µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6% of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Type IV collagen is a major macromolecular constituent of basement membranes and can be readily isolated from basement-membrane-rich tissues or highly vascularized tissues such as the placental villi. This collagen appears to be largely restricted to structures identifiable as basement membranes. In contrast, type VI collagen appears to be prevalent in several tissues even though it has been isolated largely from placental villi preparations. The extent to which type VII and VIII collagens are distributed is not known.
(). Increasing the level of cytoskeletal protein Flightless I reduces adhesion formation in a murine digital flexor tendon model. J Orthop Surg Res.
Applications
IHC, ICC, Histology

What were the immunogens for the collagen antibodies?

The immunogens for the collagen antibodies are the respective purified solubilized native collagen proteins (tropocollagens).

In what format are the collagen antibodies provided?

The collagen antibodies are provided in liquid format at a concentration of around 1 mg/mL (Absorbance 280 nm).

In what buffer are the collagen antibodies provided?

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide.

Are the antibodies specific to the reduced or non-reduced (native) proteins?

The collagen antibodies are specific to the native 3D epitopes found on the collagen native collagen protein. The collagen antibodies may have no reactivity to reduced collagen.

How was the reactivity of the antibodies tested? Was cross-reactivity to other collagens tested?

Each antibody was tested by immunoblot against each type of collagen (Type I – VI). Due to the close association of collagen proteins, cross-reactivity to other collagens may be observed. The optimal experimental conditions should be determined to minimize cross-reactivity in your application.

What are the recommended storage conditions for collagen antibodies?

The recommended storage temperature for collagen antibodies is -20°C. Please be sure to add glycerol 1:1 before freezing. We recommend making aliquots for best stability. The antibodies are stable for several weeks at 4°C.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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