Collagen Type I Antibody Biotin Conjugated
FLOW Cytometry of Anti Collagen Type I Biotin Conjugated
Flow Cytometry - Anti-Collagen 1 Biotin
Flow cytometry of Anti-Collagen Type I Antibody Biotin Conjugate
Flow Cytometry of Anti-Collagen Type I Antibody Biotin Conjugate
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Datasheet

Collagen Type I Antibody Biotin Conjugated

Rabbit Polyclonal

17 References
600-406-103
100 µg
Lyophilized
WB, ELISA, IHC, IF, FC, Dot Blot, Multiplex
Human, Mouse, Rat
Rabbit
Collagen Type I Antibody Collagen Type I Antibody Fluorescein Conjugated Collagen Type I Antibody Peroxidase Conjugated
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Product Details

Anti-Collagen Type I (RABBIT) Antibody Biotin Conjugated - 600-406-103
rabbit anti-collagen type I antibody biotin conjugation, biotin conjugated rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1
Rabbit
Biotin
Polyclonal
IgG

Target Details

COL1A1  - View All COL1A1 Products
Human, Mouse, Rat
Native Protein
Collagen Type I from human and bovine placenta.
This product has been prepared by immunoaffinity chromatography using immobilized antigens.  Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.  This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
P02452 - UniProtKB
NP_000079.2 - NCBI Protein
1277 - Gene ID

Application Details

Dot Blot, FC, Multiplex
ELISA, IF, IHC, WB  - View References
Anti-COLLAGEN Type I Antibody Biotin Conjugated has been tested by dot blot and Flow Cytometry and is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
COLLAGEN Type I Antibody Biotin Conjugated is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.
(). Beta-Sitosterol Alters Collagen Distribution in Prostate Fibroblasts. J Diet Suppl.
Applications
IF, Confocal Microscopy
(). Inhibition of lung microbiota-derived proapoptotic peptides ameliorates acute exacerbation of pulmonary fibrosis. Nature Communications
Applications
EIA, ELISA
(). The IL-4/IL-13 signaling axis promotes prostatic fibrosis. PLoS One.
Applications
IF, Confocal Microscopy
(). Blockade of PD-1/PD-L1 Pathway Enhances the Antigen-Presenting Capacity of Fibrocytes. J Immunol.
Applications
FC, FACS, FLOW
(). Fibrocytes: A Critical Review and Practical Guide. Frontiers in Immunology
Applications
IHC, ICC, Histology; FC, FACS, FLOW
(). A high-content, in vitro cardiac fibrosis assay for high-throughput, phenotypic identification of compounds with anti-fibrotic activity. J Mol Cell Cardiol.
Applications
EIA, ELISA
(). Effect of Orento, a Traditional Japanese Medicine, on IL-6, IL-8 Secretion, Type 1 Collagen Production and Alkaline Phosphatase Secretion in the Human Osteosarcoma Cell Line Saos-2. Medicines (Basel, Switzerland)
Applications
EIA, ELISA
(). Monocyte-derived fibrocytes elimination had little contribution on liver fibrosis. Hepatobiliary Pancreat Dis Int.
Applications
FC, FACS, FLOW
(). S100A4 protein is essential for the development of mature microfold cells in Peyer's patches. Cell Rep.
Applications
IHC, ICC, Histology
(). Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis. Respiratory Research
Applications
FC, FACS, FLOW
(). Vaccinia vaccine-based immunotherapy arrests and reverses established pulmonary fibrosis. JCI Insight
Applications
IHC, ICC, Histology
(). Cortactin promotes exosome secretion by controlling branched actin dynamics. Journal of Cell Biology
Applications
WB, IB, PCA
(). MicroRNA-29c regulates apoptosis sensitivity via modulation of the cell-surface death receptor, Fas, in lung fibroblasts. Am J Physiol Lung Cell Mol Physiol.
Applications
WB, IB, PCA
(). ZBTB17 (MIZ1) Is Important for the Cardiac Stress Response and a Novel Candidate Gene for Cardiomyopathy and Heart Failure. Circulation: Cardiovascular Genetics
Applications
IF, Confocal Microscopy
(). Fibrocytes represent a novel MDSC subset circulating in patients with metastatic cancer. Blood
Applications
FC, FACS, FLOW
(). CXC-type chemokines promote myofibroblast phenoconversion and prostatic fibrosis. PLoS One.
Applications
IF, Confocal Microscopy
(). Bone marrow–derived progenitor cells in pulmonary fibrosis. Journal of Clinical Investigation
Applications
IHC, ICC, Histology; FC, FACS, FLOW

What were the immunogens for the collagen antibodies?

The immunogens for the collagen antibodies are the respective purified solubilized native collagen proteins (tropocollagens).

In what format are the collagen antibodies provided?

The collagen antibodies are provided in liquid format at a concentration of around 1 mg/mL (Absorbance 280 nm).

In what buffer are the collagen antibodies provided?

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide.

Are the antibodies specific to the reduced or non-reduced (native) proteins?

The collagen antibodies are specific to the native 3D epitopes found on the collagen native collagen protein. The collagen antibodies may have no reactivity to reduced collagen.

How was the reactivity of the antibodies tested? Was cross-reactivity to other collagens tested?

Each antibody was tested by immunoblot against each type of collagen (Type I – VI). Due to the close association of collagen proteins, cross-reactivity to other collagens may be observed. The optimal experimental conditions should be determined to minimize cross-reactivity in your application.

What are the recommended storage conditions for collagen antibodies?

The recommended storage temperature for collagen antibodies is -20°C. Please be sure to add glycerol 1:1 before freezing. We recommend making aliquots for best stability. The antibodies are stable for several weeks at 4°C.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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