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Datasheet

Choline Oxidase Antibody

Goat Polyclonal

200-101-094 200-101-094S 200-101-094-0100
50 mg 25 µL 100 µg
Lyophilized Liquid (sterile filtered) Lyophilized
WB
Alcaligenes sp.
Goat
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$125.00 /Per Item
$475.00 /Per Item
50 mg $0.00
25 µL $125.00
100 µg $475.00
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Product Details

Anti-Choline Oxidase (Alcaligenes species) (GOAT) Antibody (BULK ORDER) - 200-101-094
goat anti-Choline Oxidase Antibody, codA antibody
Goat
Polyclonal
IgG

Target Details

CHOX_ALCSP  - View All CHOX_ALCSP Products
Alcaligenes sp.
Native Protein
Choline Oxidase [Alcaligenes species]
Anti-Choline Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species].  Cross reactivity against Choline Oxidase from other sources is unknown.
P16101 - UniProtKB
116367 - NCBI Protein

Application Details

WB
Choline Oxidase Antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Choline Oxidase [Alcaligenes species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:22,000 of the reconstitution concentration is suggested for this product.

Formulation

10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
5.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
Choline Oxidase catalytic activity is Choline + 2 O2 + H2O = betaine + 2 H2O2. This protein is involved in step 1 of the subpathway that synthesizes betaine from choline.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.