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Che-1 Phospho S477 (AATF) Antibody
Rabbit Polyclonal
600-401-W99
100 µL
Liquid (sterile filtered)
WB
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Che-1 pS477 (AATF) (RABBIT) Antibody - 600-401-W99
Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1, Che-1
Rabbit
Polyclonal
IgG
Target Details
AATF - View All AATF Products
Human
Phosphorylation
Conjugated Peptide
Anti-Phospho- Ser477 Che-1 (AATF) Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 of human Che-1 conjugated to KLH.
Anti-Phospho- Ser477 Che-1 (AATF) Antibody is directed against AATF protein phosphorylated at S477. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated AATF. Minimal reactivity occurs against non-phosphorylated AATF. Reactivity against AATF occurs from human sources. However, reactivity is also expected against bovine, canine, mouse, non-human primate, rat, sheep, and Xenopus based on 100% sequence homology.
Application Details
WB
Anti-Phospho-Ser477 Che-1 (AATF) Antibody is tested for Western Blots and is specific for the ~66 kDa Che-1 protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to generate the antibody but not by the corresponding dephospho-peptide. Specific conditions for reactivity should be optimized by the end user.
Formulation
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
None
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Shipping & Handling
Wet Ice
Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression. It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species. The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage. Che-1 pS477 Antibody is ideal for researchers interested in cell cycle research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
