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ChAT (Choline Acetyltransferase) Antibody
Goat Polyclonal
1 References
600-101-D16
100 µL
Liquid
WB, IHC
Human, Mouse, Avian, Chicken, Guinea Pig, Non-Human Primates
Goat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-ChAT (Choline Acetyltransferase) (GOAT) Antibody - 600-101-D16
acetyl CoA:choline O-acetyltransferase, CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, CLAT, CMS1A, CMS1A2
Goat
Polyclonal
IgG
Target Details
CHAT - View All CHAT Products
Human, Mouse, Avian, Chicken, Guinea Pig, Non-Human Primates
Native Protein
Anti-ChAT Antibody was produced in rabbit by repeated immunizations with native choline acetyltransferase purified from human placenta.
Anti-ChAT antibody is directed against human ChAT protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Cross reactivity with ChAT from other species has not been determined. However, reactivity is also expected from the following species based on 100% sequence homology: chicken, guinea pig, mouse, and non-human primates.
P28329.4 - UniProtKB
Application Details
IHC, WB
Anti-ChAT antibody is tested by western blot and immunohistochemistry and suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a ~ 68/70 kDa band corresponding to choline acetyltransferase protein by Western Blot in the appropriate lysate or tissue.
Formulation
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
5mg BSA
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
ChAT antibody recognizes choline acetyltransferase which is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. ChAT is found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome. Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects. It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function. Anti-ChAT antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Liu et al. (2017). Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep. Nature
Applications
IHC, ICC, Histology
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
