Datasheet

CDC42 Antibody

Name: CDC42 Antibody
Brand: Rockland Immunochemicals
Price: 445.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 600-401-P94

Size: 100 µg

Physical State: Lyophilized

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Host Species: Rabbit

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Specifications for CDC42 Antibody

Product Details

Description: Anti-CDC42 (RABBIT) Antibody - 600-401-P94

Synonyms: G25K GTP-binding protein

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: CDC42 - View All CDC42 Products

Reactivity: Human, Mouse, Rat, Bovine

Immunogen Type: Conjugated Peptide

Immunogen: CDC42 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human CDC42.

Purity/Specificity: Anti-CDC42 antibody is directed against human CDC42 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, and rat based on homology for the immunogen sequence. Cross reactivity with CDC42 from other sources has not been determined.

Database Links:

P60953 - UniProtKB

NP_001034891.1 - NCBI Protein

998 - Gene ID

Application Details

Tested Applications: IHC, WB

Application Note: Anti-CDC42 is tested for Immunohistochemistry -P and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~21.3 kDa corresponding to the appropriate cell lysate or extract.

Formulation

Concentration: 0.5 mg/mL by UV absorbance at 280 nm

Buffer: 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3

Preservative: 0.05mg Thimerosal

Stabilizer: 5mg BSA

Reconstitution Volume: 100 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Cell division control protein 42 homolog also known as CDC42 is a protein involved in regulation of the cell cycle. In humans, CDC42 is encoded by the CDC42 gene.CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. This antibody is suitable for researchers interested in cell signaling, epigenetics an cancer research.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Heat-induced Antigen Retrieval Protocol

Histone Immunoblotting Protocol

Immunohistochemistry (IHC) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Protease-induced Antigen Retrieval Protocol

Staining Paraffin Sections by PAP Procedure

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.