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CDC27 phospho S426 Antibody
Rabbit Polyclonal
600-401-866
100 µg
Liquid (sterile filtered)
WB, ELISA
Human, Rat, Chicken, Chimpanzee, Dog
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-CDC27 pS426 (RABBIT) Antibody - 600-401-866
rabbit anti-CDC27 pS426 Antibody, CDC 27, CDC-27, Cell division cycle protein 27 homolog, H-NUC, APC3, Anaphase-promoting complex subunit 3, ANAPC3
Rabbit
Polyclonal
IgG
Target Details
CDC27, D0S1430E - View All CDC27, D0S1430E Products
Human, Rat, Chicken, Chimpanzee, Dog
Phosphorylation
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of Human cdc27 amino acids 415-445 surrounding S426.
This affinity purified antibody is directed against the phosphorylated form of human CDC27 at the pS426 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human CDC27 pS426 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human CDC27 is minimal by ELISA. The antibody does not cross-react with CDC27 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, chicken and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with CDC27 protein from mouse may occur as sequence homology varies by one amino acid residue in this sequence (89% homology). Cross reactivity with CDC27 homologues from other sources has not been determined.
Application Details
ELISA, WB
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to CDC27 by western blotting in the appropriate cell lysate or extract. Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS426 of CDC27.
Formulation
.99 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
