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CD8a Phycoerythrin Antibody
Mouse Monoclonal OKT8 IgG2a
200-308-N62
500 µL
Liquid (sterile filtered)
FC
Human
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-CD8a (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-308-N62
T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a
Mouse
R-Phycoerythrin (RPE)
Monoclonal
IgG2a
Target Details
CD8A - View All CD8A Products
Human
Anti-CD8a Antibody (Monoclonal) was produced by repeated immunizations with CD8a antigen.
Phycoerythrin conjugated CD8a Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD8a. Reactivity is observed against human CD8a. Cross reactivity with CD8a from other sources has not been tested. Anti-CD8a is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.
Application Details
FC
Anti-CD8a is tested in Flow Cytometry and is useful for Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.
Formulation
0.012 mg/mL Sufficient to run approximately 100 tests
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.09% (w/v) Sodium Azide
0.1% Gelatin
Shipping & Handling
Wet Ice
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Expiration date is six (6) months from date of receipt.
The OKT8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites. The OKT8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
