Flow Cytometry of anti-CD8a APC - 200-526-N77

Datasheet

CD8a Allophycocyanin Antibody

Name: CD8a Allophycocyanin Antibody
Brand: Rockland Immunochemicals
Price: 369.0 USD
Availability: OutOfStock

Rat Monoclonal 53-6.7 IgG2a kappa

Item No. 200-526-N77

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Mouse

Host Species: Rat

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Specifications for CD8a Allophycocyanin Antibody

Product Details

Description: Anti-CD8a (RAT) Allophycocyanin Conjugated Monoclonal Antibody - 200-526-N77

Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Cd8a, Lyt-2, Lyt2

Host Species: Rat

Conjugate: Allophycocyanine (APC)

Clonality: Monoclonal

Format: IgG2a

Target Details

Gene Name: Cd8a - View All Cd8a Products

Reactivity: Mouse

Immunogen: Anti-CD8a Antibody (Monoclonal) was produced by repeated immunizations with CD8a antigen.

Purity/Specificity: Allophycocyanin conjugated CD8a Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD8a. Cross reactivity with CD8a from other sources has not been tested. Anti-CD8a is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.

Database Links:

P01731 - UniProtKB

NP_001074579.1 - NCBI Protein

12525 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD8a is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: 0.2mg/ml by UV absorbance at 280 nm

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: 0.1% Gelatin

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiration: Expiration date is six (6) months from date of receipt.

Background

CD8a antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites. The 53-6.7 antibody is widely used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.